PMID- 21212399
OWN - NLM
STAT- MEDLINE
DCOM- 20110407
LR  - 20110217
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Linking)
VI  - 31
IP  - 3
DP  - 2011 Mar
TI  - Neural crest-derived stem cells migrate and differentiate into cardiomyocytes 
      after myocardial infarction.
PG  - 582-9
LID - 10.1161/ATVBAHA.110.214726 [doi]
AB  - OBJECTIVE: We recently demonstrated that primitive neural crest-derived (NC) 
      cells migrate from the cardiac neural crest during embryonic development and 
      remain in the heart as dormant stem cells, with the capacity to differentiate 
      into various cell types, including cardiomyocytes. Here, we examined the 
      migration and differentiation potential of these cells on myocardial infarction 
      (MI). METHODS AND RESULTS: We obtained double-transgenic mice by crossing 
      protein-0 promoter-Cre mice with Floxed-enhanced green fluorescent protein mice, 
      in which the NC cells express enhanced green fluorescent protein. In the neonatal 
      heart, NC stem cells (NCSCs) were localized predominantly in the outflow tract, 
      but they were also distributed in a gradient from base to apex throughout the 
      ventricular myocardium. Time-lapse video analysis revealed that the NCSCs were 
      migratory. Some NCSCs persisted in the adult heart. On MI, NCSCs accumulated at 
      the ischemic border zone area (BZA), which expresses monocyte chemoattractant 
      protein-1 (MCP-1). Ex vivo cell migration assays demonstrated that MCP-1 induced 
      NCSC migration and that this chemotactic effect was significantly depressed by an 
      anti-MCP-1 antibody. Small NC cardiomyocytes first appeared in the BZA 2 weeks 
      post-MI and gradually increased in number thereafter. CONCLUSIONS: These results 
      suggested that NCSCs migrate into the BZA via MCP-1/CCR2 signaling and contribute 
      to the provision of cardiomyocytes for cardiac regeneration after MI.
FAU - Tamura, Yuichi
AU  - Tamura Y
AD  - Department of Cardiology, Keio University School of Medicine, Tokyo, Japan.
FAU - Matsumura, Keisuke
AU  - Matsumura K
FAU - Sano, Motoaki
AU  - Sano M
FAU - Tabata, Hidenori
AU  - Tabata H
FAU - Kimura, Kensuke
AU  - Kimura K
FAU - Ieda, Masaki
AU  - Ieda M
FAU - Arai, Takahide
AU  - Arai T
FAU - Ohno, Yohei
AU  - Ohno Y
FAU - Kanazawa, Hideaki
AU  - Kanazawa H
FAU - Yuasa, Shinsuke
AU  - Yuasa S
FAU - Kaneda, Ruri
AU  - Kaneda R
FAU - Makino, Shinji
AU  - Makino S
FAU - Nakajima, Kazunori
AU  - Nakajima K
FAU - Okano, Hideyuki
AU  - Okano H
FAU - Fukuda, Keiichi
AU  - Fukuda K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110106
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Myelin P0 Protein)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Wnt1 Protein)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.7.7.- (Cre recombinase)
RN  - EC 2.7.7.- (Integrases)
RN  - EC 3.4.21.- (Plasminogen Activators)
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - *Cell Lineage
MH  - *Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Disease Models, Animal
MH  - Green Fluorescent Proteins/genetics
MH  - Integrases/genetics
MH  - Mice
MH  - Mice, Transgenic
MH  - Myelin P0 Protein/genetics
MH  - Myocardial Infarction/metabolism/*pathology
MH  - Myocytes, Cardiac/metabolism/*pathology
MH  - Neural Crest/*embryology
MH  - Plasminogen Activators/genetics
MH  - Promoter Regions, Genetic
MH  - RNA Interference
MH  - Receptors, CCR2/genetics/metabolism
MH  - Regeneration
MH  - Signal Transduction
MH  - Stem Cells/metabolism/*pathology
MH  - Time Factors
MH  - Video Recording
MH  - Wnt1 Protein/genetics
EDAT- 2011/01/08 06:00
MHDA- 2011/04/08 06:00
CRDT- 2011/01/08 06:00
PHST- 2011/01/08 06:00 [entrez]
PHST- 2011/01/08 06:00 [pubmed]
PHST- 2011/04/08 06:00 [medline]
AID - ATVBAHA.110.214726 [pii]
AID - 10.1161/ATVBAHA.110.214726 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2011 Mar;31(3):582-9. doi: 
      10.1161/ATVBAHA.110.214726. Epub 2011 Jan 6.