PMID- 21213211
OWN - NLM
STAT- MEDLINE
DCOM- 20111129
LR  - 20171116
IS  - 1097-0215 (Electronic)
IS  - 0020-7136 (Linking)
VI  - 129
IP  - 11
DP  - 2011 Dec 1
TI  - miR-223 suppresses differentiation of tumor-induced CD11b(+) Gr1(+) myeloid-derived 
      suppressor cells from bone marrow cells.
PG  - 2662-73
LID - 10.1002/ijc.25921 [doi]
AB  - Tumor-associated factors are related to increased accumulation of CD11b(+) Gr1(+) 
      myeloid-derived suppressor cells (MDSCs). However, the exact mechanism of how 
      genetic factors control the expansion of MDSCs in tumor-bearing hosts remains 
      elusive. Herein, we found that tumor-associated MDSCs and their subsets, 
      mononuclear MDSCs and polymorphonuclear MDSCs, have decreased expression of 
      miR-223 when compared to CD11b(+) Gr1(+) cells from the spleen of disease-free 
      mice. With the differentiation of CD11b(+) Gr1(+) MDSCs from bone marrow cells 
      (BMCs) upon exposure to tumor-associated factors, the expression of both 
      pri-miR-223 and mature miR-223 was downregulated, indicating that the expression 
      of miR-223 could be regulated by tumor-associated factors. Interestingly, miR-223 
      remarkably inhibits differentiation of BMCs into CD11b(+) Gr1(+) MDSCs in the 
      presence of tumor-associated factors by targeting myocyte enhancer factor 2C 
      (MEF2C). Using reconstituted s.c. tumor models, miR-223 also suppresses 
      accumulation of CD11b(+) Gr1(+) MDSCs, whereas its targeting molecule MEF2C 
      increases the number of MDSCs. Tumor growth is slower in mice infused by 
      miR223-engineered BMCs than in mice infused with control transfected BMCs. As 
      miR-223 and its target molecule MEF2C are highly conserved between mice and 
      humans, the modulation of miR-223 in tumor-induced CD11b(+) Gr1(+) MDSCs may 
      exert an important role in controlling the increased accumulation of CD11b(+) 
      Gr1(+) MDSCs in patients with tumor.
CI  - Copyright (c) 2011 UICC.
FAU - Liu, Qiaofei
AU  - Liu Q
AD  - Key Laboratory of Bioactive Materials, Department of Immunology, Ministry of 
      Education, Nankai University School of Medicine, Nankai University, Tianjin, 
      China.
FAU - Zhang, Miaomiao
AU  - Zhang M
FAU - Jiang, Xingran
AU  - Jiang X
FAU - Zhang, Zhiqian
AU  - Zhang Z
FAU - Dai, Lingyun
AU  - Dai L
FAU - Min, Siping
AU  - Min S
FAU - Wu, Xilong
AU  - Wu X
FAU - He, Qingsheng
AU  - He Q
FAU - Liu, Jingyi
AU  - Liu J
FAU - Zhang, Yuan
AU  - Zhang Y
FAU - Zhang, Zhujun
AU  - Zhang Z
FAU - Yang, Rongcun
AU  - Yang R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110325
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (CD11b Antigen)
RN  - 0 (Gr-1 protein, mouse)
RN  - 0 (MEF2 Transcription Factors)
RN  - 0 (MIRN223 microRNA, mouse)
RN  - 0 (Mef2c protein, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Myogenic Regulatory Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Bone Marrow Cells/*immunology
MH  - CD11b Antigen/*metabolism
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Immunoenzyme Techniques
MH  - MEF2 Transcription Factors
MH  - Macrophages/cytology/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - MicroRNAs/*physiology
MH  - Monocytes/cytology/immunology/metabolism
MH  - Myeloid Cells/*immunology/metabolism/pathology
MH  - Myogenic Regulatory Factors/antagonists & inhibitors/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, Chemokine/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/01/08 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/01/08 06:00
PHST- 2010/04/28 00:00 [received]
PHST- 2010/12/16 00:00 [revised]
PHST- 2010/12/23 00:00 [accepted]
PHST- 2011/01/08 06:00 [entrez]
PHST- 2011/01/08 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1002/ijc.25921 [doi]
PST - ppublish
SO  - Int J Cancer. 2011 Dec 1;129(11):2662-73. doi: 10.1002/ijc.25921. Epub 2011 Mar 
      25.