PMID- 21215169
OWN - NLM
STAT- MEDLINE
DCOM- 20111017
LR  - 20160818
IS  - 0529-5807 (Print)
IS  - 0529-5807 (Linking)
VI  - 39
IP  - 11
DP  - 2010 Nov
TI  - [Analysis of chromosomal abnormalities in pancreatic cancer by spectral 
      karyotyping].
PG  - 767-71
AB  - OBJECTIVE: to investigate the chromosomal characteristics of pancreatic ductal 
      adenocarcinomas by spectral karyotyping. METHODS: cytogenetic aberrations of 
      pancreatic cancer cell line P2 established from a Chinese patient was 
      investigated by spectral karyotyping (SKY). Chromosomal alterations were further 
      evaluated in 10 cases of pancreatic cancer and 10 cases of chronic pancreatitis 
      by two color fluorescence in situ hybridization (FISH) by using EGFR/CEP7 probe 
      and paraffin embedded tissue samples. RESULTS: hypotriploid and 26 chromosomal 
      aberrations were revealed in cell line P2. Recurrent chromosomal numerical 
      alterations included loss of chromosome 4, 9, 18, 19, 22, Y, 10p, 15p, 8p, 6q and 
      12p, with gain of chromosome 7 and 12q. Frequent chromosomal structural 
      abnormalities included der(9;15)(q10;q10), der(10)(3;10)(?;q26) and 
      der(12)(8;12)(?;p13). Four of 10 cases showed EGFR copy number changes by FISH. 
      CONCLUSIONS: highly complex chromosomal rearrangements occur in pancreatic 
      cancers. Additional studies of more cases are needed, including FISH analysis of 
      EGFR copy number changes, to reach a conclusion.
FAU - Yang, Zhi-Mei
AU  - Yang ZM
AD  - Department of Pathology, Peking Union Medical College Hospital, Chinese Academy 
      of Medical Sciences, Peking Union Medical College, Beijing 100730, China.
FAU - Han, Xiao-Ping
AU  - Han XP
FAU - Wu, Sha-Fei
AU  - Wu SF
FAU - Yin, Yu-Feng
AU  - Yin YF
FAU - Wang, Ke
AU  - Wang K
FAU - Gao, Jie
AU  - Gao J
FAU - Liang, Zhi-Yong
AU  - Liang ZY
FAU - Zeng, Xuan
AU  - Zeng X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Bing Li Xue Za Zhi
JT  - Zhonghua bing li xue za zhi = Chinese journal of pathology
JID - 0005331
SB  - IM
MH  - Adenocarcinoma/*genetics
MH  - Aged
MH  - Cell Line, Tumor
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosome Duplication
MH  - Female
MH  - Gene Dosage
MH  - Genes, erbB-1/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping/*methods
MH  - Male
MH  - Middle Aged
MH  - Pancreatic Neoplasms/*genetics
EDAT- 2011/01/11 06:00
MHDA- 2011/10/18 06:00
CRDT- 2011/01/11 06:00
PHST- 2011/01/11 06:00 [entrez]
PHST- 2011/01/11 06:00 [pubmed]
PHST- 2011/10/18 06:00 [medline]
PST - ppublish
SO  - Zhonghua Bing Li Xue Za Zhi. 2010 Nov;39(11):767-71.