PMID- 21216906
OWN - NLM
STAT- MEDLINE
DCOM- 20110523
LR  - 20211020
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 77
IP  - 5
DP  - 2011 Mar
TI  - Identification of cold-temperature-regulated genes in Flavobacterium 
      psychrophilum.
PG  - 1593-600
LID - 10.1128/AEM.01717-10 [doi]
AB  - Flavobacterium psychrophilum is the etiological agent of bacterial coldwater 
      disease (BCWD) and rainbow trout fry syndrome (RTFS). It causes disease primarily 
      in fresh water-reared salmonids, but other fish species can also be affected. A 
      diverse array of clinical conditions is associated with BCWD, including tail rot 
      (peduncle disease), necrotic myositis, and cephalic osteochondritis. Degradation 
      of connective and muscular tissues by extracellular proteases is common to all of 
      these presentations. There are no effective vaccines to prevent BCWD or RTFS, and 
      antibiotics are often used to prevent and control disease. To identify virulence 
      factors that might permit development of an efficacious vaccine, cDNA suppression 
      subtractive hybridization (SSH) was used to identify cold-regulated genes in a 
      virulent strain of F. psychrophilum. Genes predicted to encode a two-component 
      system sensor histidine kinase (LytS), an ATP-dependent RNA helicase, a multidrug 
      ABC transporter permease/ATPase, an outer membrane protein/protective antigen 
      OMA87, an M43 cytophagalysin zinc-dependent metalloprotease, a hypothetical 
      protein, and four housekeeping genes were upregulated at 8 degrees C versus the level of 
      expression at 20 degrees C. Because no F. psychrophilum gene was known to be suitable as 
      an internal standard in reverse transcription-quantitative real-time PCR 
      (RT-qPCR) experiments, the expression stability of nine commonly used reference 
      genes was evaluated at 8 degrees C and 20 degrees C. Expression of the 16S rRNA was equivalent at 
      both temperatures, and this gene was used in RT-qPCR experiments to verify the 
      SSH findings. With the exception of the ATCC 49513 strain, similar patterns of 
      gene expression were obtained with 11 other representative strains of F. 
      psychrophilum.
FAU - Hesami, Shohreh
AU  - Hesami S
AD  - Department of Pathobiology, University of Guelph, Guelph, ON N1G 2W1, Canada.
FAU - Metcalf, Devon S
AU  - Metcalf DS
FAU - Lumsden, John S
AU  - Lumsden JS
FAU - Macinnes, Janet I
AU  - Macinnes JI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110107
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - *Cold Temperature
MH  - Flavobacterium/*genetics/*radiation effects
MH  - *Gene Expression Profiling
MH  - *Gene Expression Regulation, Bacterial
MH  - Gene Library
MH  - *Genes, Bacterial
MH  - Nucleic Acid Hybridization
MH  - RNA, Ribosomal, 16S/genetics
MH  - *Stress, Physiological
MH  - Virulence Factors/biosynthesis
PMC - PMC3067270
EDAT- 2011/01/11 06:00
MHDA- 2011/05/24 06:00
PMCR- 2011/09/01
CRDT- 2011/01/11 06:00
PHST- 2011/01/11 06:00 [entrez]
PHST- 2011/01/11 06:00 [pubmed]
PHST- 2011/05/24 06:00 [medline]
PHST- 2011/09/01 00:00 [pmc-release]
AID - AEM.01717-10 [pii]
AID - 1717-10 [pii]
AID - 10.1128/AEM.01717-10 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2011 Mar;77(5):1593-600. doi: 10.1128/AEM.01717-10. Epub 
      2011 Jan 7.