PMID- 2121851
OWN - NLM
STAT- MEDLINE
DCOM- 19901213
LR  - 20161123
IS  - 0197-8357 (Print)
IS  - 0197-8357 (Linking)
VI  - 10
IP  - 4
DP  - 1990 Aug
TI  - Basal and interferon-induced 2',5'-oligoadenylate synthetase in human monocytes, 
      lymphocytes, and peritoneal macrophages.
PG  - 393-402
AB  - 2',5'-Oligoadenylate (2-5A) synthetase is an intracellular enzyme induced during 
      viral diseases and after clinical administration of interferons (IFN). Because 
      monocytes and T and B lymphocytes in peripheral blood are targets of viral 
      infection, we compared the basal levels and induction of 2-5A synthetase activity 
      in these three cell types. IFN-beta induced significant, three- to eightfold 
      increases in 2-5A synthetase activity in monocytes and T and B lymphocytes, both 
      in vitro and in vivo. IFN-gamma was less effective in inducing 2-5A synthetase 
      than IFN-beta, yielding up to 2.6-fold increases in monocytes and slight 
      increases in lymphocytes in vitro. IFN-beta also had greater 2-5A 
      synthetase-inducing activity than IFN-gamma in HL-60 and U937 monocytic cell 
      lines and in human peritoneal macrophages. Baseline levels of 2-5A synthetase in 
      untreated peripheral blood monocytes separated by adherence or density gradients 
      were three- to fivefold higher than in lymphocytes. 2-5A synthetase levels were 
      twofold higher in B than in T lymphocytes separated by fluorescence-activated 
      cell sorting. Tumor necrosis factor-alpha (TNF-alpha) did not induce this enzyme 
      in peripheral blood mononuclear cells (PBMCs) or peritoneal macrophages. Thus, 
      monocytes had greater basal and induced levels of 2-5A synthetase activity than 
      did lymphocytes and contributed disproportionately to total 2-5A synthetase 
      activity in peripheral blood.
FAU - Witt, P L
AU  - Witt PL
AD  - Department of Human Oncology, University of Wisconsin Clinical Cancer Center, 
      Madison 53792.
FAU - Spear, G T
AU  - Spear GT
FAU - Helgeson, D O
AU  - Helgeson DO
FAU - Lindstrom, M J
AU  - Lindstrom MJ
FAU - Smalley, R V
AU  - Smalley RV
FAU - Borden, E C
AU  - Borden EC
LA  - eng
GR  - N01-CM-47669-05/CM/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Interferon Res
JT  - Journal of interferon research
JID - 8100396
RN  - 0 (Interferon Type I)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 145155-23-3 (Interferon beta-1b)
RN  - 77238-31-4 (Interferon-beta)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.7.84 (2',5'-Oligoadenylate Synthetase)
RN  - XRO4566Q4R (Interferon beta-1a)
SB  - IM
MH  - 2',5'-Oligoadenylate Synthetase/*biosynthesis
MH  - Cell Separation
MH  - Enzyme Induction
MH  - Flow Cytometry
MH  - Humans
MH  - In Vitro Techniques
MH  - Interferon Type I/*pharmacology
MH  - Interferon beta-1a
MH  - Interferon beta-1b
MH  - *Interferon-beta
MH  - Interferon-gamma/*pharmacology
MH  - Kinetics
MH  - Lymphocytes/*drug effects/enzymology
MH  - Macrophages/*drug effects/enzymology
MH  - Monocytes/*drug effects/enzymology
MH  - Peritoneal Cavity/cytology
MH  - Recombinant Proteins/pharmacology
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 10.1089/jir.1990.10.393 [doi]
PST - ppublish
SO  - J Interferon Res. 1990 Aug;10(4):393-402. doi: 10.1089/jir.1990.10.393.