PMID- 21220748
OWN - NLM
STAT- MEDLINE
DCOM- 20110509
LR  - 20220310
IS  - 1528-0020 (Electronic)
IS  - 0006-4971 (Linking)
VI  - 117
IP  - 9
DP  - 2011 Mar 3
TI  - Ex vivo-expanded DCs induce donor-specific central and peripheral tolerance and 
      prolong the acceptance of donor skin grafts.
PG  - 2640-8
LID - 10.1182/blood-2010-07-293860 [doi]
AB  - Dendritic cells (DCs) are known to regulate immune responses by inducing both 
      central and peripheral tolerance. DCs play a vital role in negative selection of 
      developing thymocytes by deleting T cells with high-affinity for 
      self-peptide-major histocompatibility complexes. In the periphery, DCs mediate 
      peripheral tolerance by promoting regulatory T-cell development, induction of 
      T-cell unresponsiveness, and deletion of activated T cells. We studied whether 
      allogeneic DCs, obtained from bone marrow cultured with either Flt3L (FLDCs) or 
      granulocyte-macrophage colony-stimulating factor (GMDCs), could induce 
      allospecific central and peripheral tolerance after IV injection; B cells were 
      used as a control. The results showed that only FLDCs reached the thymus after 
      injection and that these cells induced both central and peripheral tolerance to 
      donor major histocompatibility complexes. For central tolerance, injection of 
      FLDCs induced antigen-specific clonal deletion of both CD8 and CD4 
      single-positive thymocytes. For peripheral tolerance, injection of FLDCs induced 
      donor-specific T-cell unresponsiveness and prolonged survival of donor-derived 
      skin grafts. Tolerance induction by adoptive transfer of FLDCs could be a useful 
      approach for promoting graft acceptance after organ transplantation.
CI  - (c) 2011 by The American Society of Hematology
FAU - Yamano, Tomoyoshi
AU  - Yamano T
AD  - Division of Immunobiology, Research Institute for Biological Sciences, Tokyo 
      University of Science, Tokyo, Japan.
FAU - Watanabe, Sho
AU  - Watanabe S
FAU - Hasegawa, Hiroyuki
AU  - Hasegawa H
FAU - Suzuki, Toshihiro
AU  - Suzuki T
FAU - Abe, Ryo
AU  - Abe R
FAU - Tahara, Hideaki
AU  - Tahara H
FAU - Nitta, Takeshi
AU  - Nitta T
FAU - Ishimaru, Naozumi
AU  - Ishimaru N
FAU - Sprent, Jonathan
AU  - Sprent J
FAU - Kishimoto, Hidehiro
AU  - Kishimoto H
LA  - eng
PT  - Journal Article
DEP - 20110110
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Epitopes)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Clone Cells
MH  - Dendritic Cells/*cytology/*immunology
MH  - Epitopes/immunology
MH  - Graft Survival/*immunology
MH  - Immune Tolerance/*immunology
MH  - Injections
MH  - Membrane Proteins/metabolism
MH  - Mice
MH  - Side-Population Cells/cytology/immunology
MH  - Skin Transplantation/*immunology
MH  - T-Lymphocytes/cytology/immunology
MH  - Thymus Gland/cytology
MH  - Time Factors
MH  - Transplantation, Homologous
EDAT- 2011/01/12 06:00
MHDA- 2011/05/10 06:00
CRDT- 2011/01/12 06:00
PHST- 2011/01/12 06:00 [entrez]
PHST- 2011/01/12 06:00 [pubmed]
PHST- 2011/05/10 06:00 [medline]
AID - S0006-4971(20)39060-1 [pii]
AID - 10.1182/blood-2010-07-293860 [doi]
PST - ppublish
SO  - Blood. 2011 Mar 3;117(9):2640-8. doi: 10.1182/blood-2010-07-293860. Epub 2011 Jan 
      10.