PMID- 21220936
OWN - NLM
STAT- MEDLINE
DCOM- 20110421
LR  - 20220310
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 26
IP  - 6
DP  - 2010
TI  - Costus pictus extracts stimulate insulin secretion from mouse and human islets of 
      Langerhans in vitro.
PG  - 1051-8
LID - 10.1159/000324007 [doi]
AB  - Plant-derived extracts have been used as folk remedies for Type 2 diabetes 
      mellitus (T2DM) for many centuries, and offer the potential of cheap and readily 
      available alternatives to conventional pharmaceuticals in developing countries. 
      Extracts of Costus pictus (CP), a plant belonging to the Costaceae family, are 
      reported to have antidiabetic activity in vivo. The exact molecular mode of 
      action(s) of CP is unclear but the antihyperglycemic effect seen in animal 
      studies was associated with dramatic increases in insulin secretion so in our 
      study we have measured the effect of aqueous CP extract on insulin secretion in 
      vitro from the MIN6 beta-cell line and isolated mouse and human islets. Our data 
      demonstrate that CP has a direct stimulatory effect on insulin secretion at basal 
      but not stimulatory glucose concentrations which was not associated with 
      compromised membrane integrity or decrease beta-cell viability. Single cell calcium 
      microfluorimetry measurements showed that CP caused elevations in beta-cell 
      intracellular Ca(2+) concentrations ([Ca(2+)](i)), an effect which was completely 
      abolished by the removal of extracellular Ca(2+) or blockade of voltage-gated 
      Ca(2+) channels (VGCC). These in vitro observations suggest that one mode of 
      action of CP is through stimulating insulin secretion which may be mediated, in 
      part, by the ability of CP to increase [Ca(2+)](i) levels through VGCC. CP 
      extracts may provide an affordable and inexpensive alternative for treating 
      patients with T2DM.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Al-Romaiyan, Altaf
AU  - Al-Romaiyan A
AD  - Diabetes Research Group, King's College London, London, United Kingdom. 
      altaf.al-romaiyan@kcl.ac.uk
FAU - Jayasri, Mangalam A
AU  - Jayasri MA
FAU - Mathew, T Lazar
AU  - Mathew TL
FAU - Huang, Guo-Cai
AU  - Huang GC
FAU - Amiel, Stephanie
AU  - Amiel S
FAU - Jones, Peter M
AU  - Jones PM
FAU - Persaud, Shanta J
AU  - Persaud SJ
LA  - eng
GR  - G0700557/1/NC3RS_/National Centre for the Replacement, Refinement and Reduction 
      of Animals in Research/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110104
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Calcium Channels)
RN  - 0 (Insulin)
RN  - 0 (Plant Extracts)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calcium Channel Blockers/pharmacology
MH  - Calcium Channels/*chemistry/metabolism
MH  - Cell Line
MH  - Costus/*chemistry
MH  - Diabetes Mellitus, Type 2/drug therapy
MH  - Humans
MH  - Insulin/*metabolism
MH  - Insulin Secretion
MH  - Islets of Langerhans/*drug effects/metabolism
MH  - Mice
MH  - Plant Extracts/chemistry/*pharmacology
EDAT- 2011/01/12 06:00
MHDA- 2011/04/22 06:00
CRDT- 2011/01/12 06:00
PHST- 2010/11/04 00:00 [accepted]
PHST- 2011/01/12 06:00 [entrez]
PHST- 2011/01/12 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - 000324007 [pii]
AID - 10.1159/000324007 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2010;26(6):1051-8. doi: 10.1159/000324007. Epub 2011 Jan 4.