PMID- 21223560
OWN - NLM
STAT- MEDLINE
DCOM- 20110429
LR  - 20211020
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 9
DP  - 2011 Jan 11
TI  - Expression of miRNAs in ovine fetal gonads: potential role in gonadal 
      differentiation.
PG  - 2
LID - 10.1186/1477-7827-9-2 [doi]
AB  - BACKGROUND: Gonadal differentiation in the mammalian fetus involves a complex 
      dose-dependent genetic network. Initiation and progression of fetal ovarian and 
      testicular pathways are accompanied by dynamic expression patterns of thousands 
      of genes. We postulate these expression patterns are regulated by small 
      non-coding RNAs called microRNAs (miRNAs). The aim of this study was to identify 
      the expression of miRNAs in mammalian fetal gonads using sheep as a model. 
      METHODS: We determined the expression of 128 miRNAs by real time PCR in 
      early-gestational (gestational day (GD) 42) and mid-gestational (GD75) sheep 
      ovaries and testes. Expression data were further examined and validated by 
      bioinformatic analysis. RESULTS: Expression analysis revealed significant 
      differences between ovaries and testes among 24 miRNAs at GD42, and 43 miRNAs at 
      GD75. Bioinformatic analysis revealed that a number of differentially expressed 
      miRNAs are predicted to target genes known to be important in mammalian gonadal 
      development, including ESR1, CYP19A1, and SOX9. In situ hybridization revealed 
      miR-22 localization within fetal testicular cords. As estrogen signaling is 
      important in human and sheep ovarian development, these data indicate that miR-22 
      is involved in repressing estrogen signaling within fetal testes. CONCLUSIONS: 
      Based on our results we postulate that gene expression networks underlying fetal 
      gonadal development are regulated by miRNAs.
FAU - Torley, Katie J
AU  - Torley KJ
AD  - Animal Reproduction and Biotechnology Laboratory, Department of Biomedical 
      Sciences, Colorado State University, Fort Collins, CO 80523, USA.
FAU - da Silveira, Juliano C
AU  - da Silveira JC
FAU - Smith, Peter
AU  - Smith P
FAU - Anthony, Russell V
AU  - Anthony RV
FAU - Veeramachaneni, D N Rao
AU  - Veeramachaneni DN
FAU - Winger, Quinton A
AU  - Winger QA
FAU - Bouma, Gerrit J
AU  - Bouma GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110111
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (MicroRNAs)
RN  - 0 (SOX9 Transcription Factor)
RN  - EC 1.14.14.1 (Aromatase)
SB  - IM
MH  - Animals
MH  - Aromatase/genetics
MH  - Estrogen Receptor alpha/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental
MH  - Gonads/*embryology
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Ovary/embryology
MH  - SOX9 Transcription Factor/genetics
MH  - Sex Differentiation/*genetics
MH  - Sheep, Domestic/genetics
MH  - Testis/embryology
PMC - PMC3027096
EDAT- 2011/01/13 06:00
MHDA- 2011/04/30 06:00
PMCR- 2011/01/11
CRDT- 2011/01/13 06:00
PHST- 2010/10/06 00:00 [received]
PHST- 2011/01/11 00:00 [accepted]
PHST- 2011/01/13 06:00 [entrez]
PHST- 2011/01/13 06:00 [pubmed]
PHST- 2011/04/30 06:00 [medline]
PHST- 2011/01/11 00:00 [pmc-release]
AID - 1477-7827-9-2 [pii]
AID - 10.1186/1477-7827-9-2 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2011 Jan 11;9:2. doi: 10.1186/1477-7827-9-2.