PMID- 21223812
OWN - NLM
STAT- MEDLINE
DCOM- 20111115
LR  - 20120621
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 90
IP  - 48
DP  - 2010 Dec 28
TI  - [Analysis of BRAF mutation and extracellular regulated protein kinases activation 
      in nasal mucosa melanomas].
PG  - 3399-402
AB  - OBJECTIVE: To determine the rate of BRAF gene mutation and the expression of 
      activated extracellular regulated protein kinases (ERK) in nasal mucosa malignant 
      melanomas and compare the results with those in cutaneous melanomas so as to 
      explore the difference in the pathogenesis between nasal mucosa malignant 
      melanomas and cutaneous melanomas. METHODS: Twenty nasal mucosa melanomas samples 
      were collected. Genomic DNA was extracted and the BRAF mutation detected by PCR 
      (polymerase chain reaction) and direct sequencing analysis. Total protein was 
      obtained and the activated ERK detected by Western blot. RESULTS: BRAF mutation 
      was detected in 5% (1/20) samples of all patients. The mutation rate was much 
      lower than that in cutaneous melanomas. And phosphorylated ERK protein was 
      detected in 60% (12/20). And the result was almost similar to that of cutaneous 
      melanomas. CONCLUSIONS: The high expression of activated ERK is not caused mainly 
      by BRAF gene mutation in nasal mucosa malignant melanomas. The pathogenic 
      pathways are different between nasal mucosa malignant melanomas and cutaneous 
      melanomas.
FAU - Li, Hou-Yong
AU  - Li HY
AD  - Department of Otorhinolaryngology, Eye & Ear Nose Throat Hospital, Fudan 
      University, Shanghai 200031, China. lihouy2000@yahoo.com.cn
FAU - Zhou, Liang
AU  - Zhou L
FAU - Tian, Jie
AU  - Tian J
FAU - Cong, Ning
AU  - Cong N
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - EC 2.7.11.1 (BRAF protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - DNA Mutational Analysis
MH  - Extracellular Signal-Regulated MAP Kinases/genetics/*metabolism
MH  - Female
MH  - Humans
MH  - Melanoma/genetics/*metabolism
MH  - Middle Aged
MH  - Mutation
MH  - Nasal Mucosa/metabolism/pathology
MH  - Nose Neoplasms/genetics/*metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins B-raf/*genetics
EDAT- 2011/01/13 06:00
MHDA- 2011/11/16 06:00
CRDT- 2011/01/13 06:00
PHST- 2011/01/13 06:00 [entrez]
PHST- 2011/01/13 06:00 [pubmed]
PHST- 2011/11/16 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2010 Dec 28;90(48):3399-402.