PMID- 21226625
OWN - NLM
STAT- MEDLINE
DCOM- 20110805
LR  - 20161125
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Linking)
VI  - 17
IP  - 9-10
DP  - 2011 May
TI  - Orthodontic tooth movement in alveolar cleft repaired with a tissue engineering 
      bone: an experimental study in dogs.
PG  - 1313-25
LID - 10.1089/ten.TEA.2010.0490 [doi]
AB  - Tissue engineering approaches have been successfully used in repairing bone 
      defects and have become a viable alternative to autologous bone. The aim of the 
      present study was to investigate if a construct of porous beta-tricalcium 
      phosphate (beta-TCP) combined with osteogenically induced bone marrow stromal cells 
      (bMSCs) could repair alveolar cleft, and allow for subsequent orthodontic tooth 
      movement in a canine model. Twelve alveolar osteotomy surgeries in six animals 
      were made bilaterally and randomly implanted by (1) tissue-engineered bone 
      complex of bMSCs/beta-TCP (group A, n=4), (2) beta-TCP alone (group B, n=4), and (3) 
      autologous bone obtained from iliac bone (group C, n=4). Contralateral alveolar 
      defects were created in one animal and left untreated to serve as blank control 
      to observe spontaneous healing of the defects. Sequential fluorescent labeling 
      and radiographic observation was used to evaluate new bone formation and 
      mineralization in each defect. Orthodontic tooth movement was initiated 8 weeks 
      after surgical operation for 12 weeks, and then the dogs were sacrificed for 
      histological and histomorphometric analysis. Results indicated that the 
      tissue-engineered complex with bMSCs/beta-TCP dramatically promoted new bone 
      formation and mineralization and achieved a favorable height of the repaired 
      alveolar when compared with beta-TCP alone, which absorbed severely. The overall 
      effect of the tissue-engineered bone was equivalent to autologous bone; the 
      physiological function of the alveolar bone was restored by allowing the adjacent 
      teeth to move into the newly formed bone in the grafted region. This study 
      demonstrated that the tissue engineering bone from the combination of beta-TCP and 
      bMSCs is a feasible clinical approach for patients with alveolar cleft and the 
      subsequent orthodontic tooth movement.
FAU - Zhang, Dongjie
AU  - Zhang D
AD  - Department of Orthodontics, Ninth People's Hospital Affiliated to Shanghai Jiao 
      Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, 
      Shanghai, China.
FAU - Chu, Fengting
AU  - Chu F
FAU - Yang, Yan
AU  - Yang Y
FAU - Xia, Lunguo
AU  - Xia L
FAU - Zeng, Deliang
AU  - Zeng D
FAU - Uludag, Hasan
AU  - Uludag H
FAU - Zhang, Xiuli
AU  - Zhang X
FAU - Qian, Yufen
AU  - Qian Y
FAU - Jiang, Xinquan
AU  - Jiang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110317
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (beta-tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*pharmacology
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Calcium Phosphates/*pharmacology
MH  - Dogs
MH  - Humans
MH  - *Osteogenesis
MH  - Porosity
MH  - Stromal Cells/cytology/metabolism
MH  - Time Factors
MH  - *Tissue Engineering
MH  - Tooth Movement Techniques/*methods
EDAT- 2011/01/14 06:00
MHDA- 2011/08/06 06:00
CRDT- 2011/01/14 06:00
PHST- 2011/01/14 06:00 [entrez]
PHST- 2011/01/14 06:00 [pubmed]
PHST- 2011/08/06 06:00 [medline]
AID - 10.1089/ten.TEA.2010.0490 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2011 May;17(9-10):1313-25. doi: 10.1089/ten.TEA.2010.0490. 
      Epub 2011 Mar 17.