PMID- 21229763
OWN - NLM
STAT- MEDLINE
DCOM- 20110623
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 31
IP  - 10
DP  - 2010 Oct
TI  - [Prokaryotic expression of human vitamin D receptor (hVDR) and its binding 
      activities to Cd and Pb].
PG  - 2469-74
AB  - Human vitamin D receptor (hVDR) is a potential receptor channel for heavy metals 
      to affect bone metabolism, while to date there is no report about the binding 
      activity between heavy metals and hVDR. This study established a prokaryotic 
      expression of hVDR system, by cloned hVDR-LBD into pGEX-4T-1 vector. Then 
      according to the principle that the nuclear receptor binding with its ligand can 
      be combined with nuclear receptor coactivator 2-bacterial alkaline phosphatase 
      fusion protein (TIF2-BAP), we established a method of 
      p-nitrophenylphosphate-alkaline phosphatase to analyse the effects of chemical on 
      the binding activity between hVDR and TIF2-BAP. Using this method, we studied the 
      binding activities between hVDR and TIF2-BAP after exposure of cadmium and lead. 
      The results showed that the binding activities significantly increased to 3.95 
      and 4.39 times that of the control after exposure of 100 micromol/L and 1 000 
      micromol/L cadmium chloride (CdCl2), and the binding activities significantly 
      increased to 2.29 and 3.52 times that of the control after exposure of 100 
      micromol/L and 1 000 micromol/L lead acetate (PbAc2), respectively. These results 
      indicate that cadmium and lead can mimic the activity of 1,25-(OH)2D3, disrupt 
      the normal function of hVDR receptor channel, which may be the underlying 
      mechanism of abnormal bone metabolism and osteoporosis caused by cadmium and 
      lead.
FAU - Jiao, Jian
AU  - Jiao J
AD  - School of Food Science and Technology, Henan University of Technology, Zhengzhou 
      450052, China. jiaojian1111@yahoo.com.cn
FAU - Zhang, Zhao-Bin
AU  - Zhang ZB
FAU - Hu, Jian-Ying
AU  - Hu JY
FAU - Wang, Jin-Shui
AU  - Wang JS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Environmental Pollutants)
RN  - 0 (NCOA2 protein, human)
RN  - 0 (Nuclear Receptor Coactivator 2)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 00BH33GNGH (Cadmium)
RN  - 2P299V784P (Lead)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Cadmium/*metabolism/toxicity
MH  - Cloning, Molecular
MH  - Environmental Pollutants/*metabolism/toxicity
MH  - Genetic Vectors/genetics
MH  - Humans
MH  - Lead/*metabolism/toxicity
MH  - Nuclear Receptor Coactivator 2/metabolism
MH  - Osteoporosis/*chemically induced
MH  - Receptors, Calcitriol/biosynthesis/genetics/*metabolism
MH  - Recombinant Fusion Proteins/biosynthesis/genetics
EDAT- 2011/01/15 06:00
MHDA- 2011/06/24 06:00
CRDT- 2011/01/15 06:00
PHST- 2011/01/15 06:00 [entrez]
PHST- 2011/01/15 06:00 [pubmed]
PHST- 2011/06/24 06:00 [medline]
PST - ppublish
SO  - Huan Jing Ke Xue. 2010 Oct;31(10):2469-74.