PMID- 2123188
OWN - NLM
STAT- MEDLINE
DCOM- 19910108
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 265
IP  - 32
DP  - 1990 Nov 15
TI  - Hyaluronic acid synthesis by mural granulosa cells and cumulus cells in vitro is 
      selectively stimulated by a factor produced by oocytes and by transforming growth 
      factor-beta.
PG  - 19517-23
AB  - In ovarian antral follicles cumulus cells (approximately 1,000/follicle) closely 
      surround the oocyte, and mural granulosa cells (approximately 50,000/follicle) 
      are distributed at the periphery. Previous work (Salustri, A., Yanagishita, M., 
      and Hascall, V. C. (1990) Dev. Biol. 138, 26-32) showed that oocytes produce a 
      factor(s) which stimulates hyaluronic acid (HA) synthesis by cumulus cells during 
      expansion of the cumulus cell-oocyte complex. We now show that mural granulosa 
      cells also respond in vitro to the oocyte factor(s) with greatly increased HA 
      synthesis. As with cumulus cells, a factor(s) present in fetal calf serum is 
      required to retain newly synthesized HA in the extracellular matrix. Unlike 
      cumulus cells, follicle-stimulating hormone (FSH) is not required for maximal 
      stimulation, in part because mural granulosa cells synthesize prostaglandin E2 
      which can substitute for FSH in promoting cumulus cell-oocyte complex expansion. 
      Of several growth factors studied, only transforming growth factor-beta 1 
      (TGF-beta 1) stimulated HA synthesis in both cell types. However, the stimulation 
      of HA synthesis by TGF-beta 1 was additive with that for the oocyte factor(s), 
      and neutralizing antibodies to TGF-beta did not inhibit the response to the 
      oocyte factor(s). The results indicate that the oocyte factor(s) and TGF-beta 1 
      are not the same and that they operate through different receptors in stimulating 
      HA synthesis. Epidermal growth factor was able to replace FSH in amplifying the 
      response of cumulus cells to the oocyte factor(s) and in stimulating synthesis of 
      dermatan sulfate proteoglycans.
FAU - Salustri, A
AU  - Salustri A
AD  - Department of Sanita Pubblica e Biologia Cellulare, Faculty of Medicine, 2nd 
      University of Rome, Italy.
FAU - Ulisse, S
AU  - Ulisse S
FAU - Yanagishita, M
AU  - Yanagishita M
FAU - Hascall, V C
AU  - Hascall VC
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Growth Substances)
RN  - 0 (Transforming Growth Factor beta)
RN  - 24967-94-0 (Dermatan Sulfate)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Dermatan Sulfate/biosynthesis
MH  - Dinoprostone/pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Granulosa Cells/drug effects/*metabolism
MH  - Growth Substances/biosynthesis/*pharmacology
MH  - Hyaluronic Acid/*biosynthesis
MH  - Kinetics
MH  - Mice
MH  - Oocytes/*metabolism
MH  - Ovarian Follicle/drug effects/*metabolism
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1990/11/15 00:00
MHDA- 1990/11/15 00:01
CRDT- 1990/11/15 00:00
PHST- 1990/11/15 00:00 [pubmed]
PHST- 1990/11/15 00:01 [medline]
PHST- 1990/11/15 00:00 [entrez]
AID - S0021-9258(17)45403-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1990 Nov 15;265(32):19517-23.