PMID- 21232224
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110114
IS  - 1009-3419 (Print)
IS  - 1009-3419 (Linking)
VI  - 7
IP  - 3
DP  - 2004 Jun 20
TI  - [Detection of CK19 and CEA mRNA expression for the diagnosis of peripheral blood 
      micrometastases in patients with non-small cell lung cancer].
PG  - 226-9
LID - 10.3779/j.issn.1009-3419.2004.03.11 [doi]
AB  - BACKGROUND: To explore the sensitivity and clinical significance of detection of 
      CK19 mRNA and CEA mRNA expression for the diagnosis of micrometastases in the 
      peripheral blood of patients with non small cell lung cancer (NSCLC). METHODS: 
      Nested reverse transcription-polymerase chain reaction (RT-PCR) was used to 
      detect CK19 mRNA and CEA mRNA in the peripheral blood of 35 patients with NSCLC. 
      Twenty patients with pulmonary benign lesions and 20 normal healthy volunteers 
      were served as controls. RESULTS: The positive rates of CK19 mRNA and CEA mRNA 
      for NSCLC patients were 40% (14/35) and 57% (20/35) respectively, 14 cases (40%) 
      were positive for both mRNA, and 15 cases (43%) were both negative for both mRNA. 
      No blood samples from patients with pulmonary benign lesions was positive for CEA 
      mRNA, but 2 samples (10%) were positive for CK19 mRNA. Of these 20 samples from 
      normal volunteers, none was positive for both CK19 mRNA and CEA mRNA. The distant 
      metastasis rate of patients with positive CK-19 mRNA , positive CEA mRNA and both 
      negative in the peripheral blood were 50%, 56% and 27% respectively. CONCLUSIONS: 
      Both CK-19 mRNA and CEA mRNA are good markers to detect micrometastasis in the 
      peripheral blood of patients with NSCLC. Detection of CK19 and CEA mRNA 
      expression might be helpful to predict the prognosis of patients with lung 
      cancer.
FAU - Zhu, Guangying
AU  - Zhu G
AD  - Department of Radiation Oncology, Cancer Institute, Xuzhou Medical College, 
      Xuzhou, Jiangsu 221002, P.R.China.
FAU - Liu, Delin
AU  - Liu D
FAU - Wang, Xu
AU  - Wang X
FAU - Chen, Jie
AU  - Chen J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
EDAT- 2004/06/20 00:00
MHDA- 2004/06/20 00:01
CRDT- 2011/01/15 06:00
PHST- 2011/01/15 06:00 [entrez]
PHST- 2004/06/20 00:00 [pubmed]
PHST- 2004/06/20 00:01 [medline]
AID - 10.3779/j.issn.1009-3419.2004.03.11 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2004 Jun 20;7(3):226-9. doi: 
      10.3779/j.issn.1009-3419.2004.03.11.