PMID- 21232748
OWN - NLM
STAT- MEDLINE
DCOM- 20110713
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1218
IP  - 15
DP  - 2011 Apr 15
TI  - Kinetic behaviour in supercritical fluid chromatography with modified mobile 
      phase for 5 mum particle size and varied flow rates.
PG  - 2058-64
LID - 10.1016/j.chroma.2010.12.057 [doi]
AB  - After much development of stationary phase chemistry, in recent years the focus 
      of many studies in HPLC has shifted to increase the efficiency and analysis 
      speed. Ultra high pressure liquid chromatography (UHPLC) using sub-2 mum 
      particles, and high temperature liquid chromatography (HTLC), using temperatures 
      above 100 degrees C have received much attention. These new approaches allow the use of 
      flow rates higher than those classically used in HPLC, reducing the analysis 
      duration. Due to the low viscosity of supercritical fluids, high velocities, i.e. 
      high flow rates, can be achieved with classical pumping systems typically used in 
      supercritical fluid chromatography (SFC). The effects of the flow rate increase 
      with CO(2)/methanol mobile phase was studied on the inlet pressure, t(0), the 
      retention factor of the compounds, and on the efficiency. Simple comparisons of 
      efficiencies obtained at varied temperature between SFC and HPLC, with a packed 
      column containing 5 mum particles, show the greater kinetic performances achieved 
      with the CO(2)/methanol fluid, and underline specific behaviours of SFC, 
      occurring for high flow rates and sub-ambient temperature. Some values (N/t(0)) 
      are also compared to UHPLC data, showing that good performance can be achieved in 
      SFC without applying drastic analytical conditions. Finally, simple kinetic plots 
      (t(0) vs N) at constant column length are used to select combinations of 
      temperature and flow rate necessary to achieve a required theoretical plate 
      number.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Lesellier, E
AU  - Lesellier E
AD  - ICOA, UFR Science, UMR 6005, BP 6759, rue de Chartres, 45067 Orleans cedex 2, 
      France. eric.lesellier@univ-orleans.fr
FAU - Fougere, L
AU  - Fougere L
FAU - Poe, Donald P
AU  - Poe DP
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20101221
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 142M471B3J (Carbon Dioxide)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Carbon Dioxide/chemistry
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Supercritical Fluid/*methods
MH  - Kinetics
MH  - Methanol/chemistry
MH  - Particle Size
MH  - Temperature
EDAT- 2011/01/15 06:00
MHDA- 2011/07/14 06:00
CRDT- 2011/01/15 06:00
PHST- 2010/07/29 00:00 [received]
PHST- 2010/12/09 00:00 [revised]
PHST- 2010/12/14 00:00 [accepted]
PHST- 2011/01/15 06:00 [entrez]
PHST- 2011/01/15 06:00 [pubmed]
PHST- 2011/07/14 06:00 [medline]
AID - S0021-9673(10)01749-8 [pii]
AID - 10.1016/j.chroma.2010.12.057 [doi]
PST - ppublish
SO  - J Chromatogr A. 2011 Apr 15;1218(15):2058-64. doi: 10.1016/j.chroma.2010.12.057. 
      Epub 2010 Dec 21.