PMID- 21233831
OWN - NLM
STAT- MEDLINE
DCOM- 20110609
LR  - 20151119
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Linking)
VI  - 25
IP  - 4
DP  - 2011 Apr
TI  - The isotype of the BCR as a surrogate for the GCB and ABC molecular subtypes in 
      diffuse large B-cell lymphoma.
PG  - 681-8
LID - 10.1038/leu.2010.302 [doi]
AB  - Gene expression profiling has identified two major molecular subtypes of diffuse 
      large B-cell lymphoma (DLBCL) that are histologically indistinguishable but 
      differ in cure rates. Here, we investigated whether the isotype of the B-cell 
      receptor (BCR) expressed by the tumoral cells correlated with the molecular 
      subtype and survival. Gene expression analysis clustered the 53 patients included 
      in this study into three subgroups, 17 germinal center B-cell-like (GCB) cases, 
      26 activated B-cell-like (ABC) cases and 10 intermediate cases. The molecular 
      subtype was correlated with the isotype, as 15/17 GCB cases expressed a secondary 
      isotype (immunoglobulin (Ig)G or IgA), whereas 24/26 ABC cases expressed a 
      primary isotype (IgM or IgD) (P<0.0001). There was a trend toward a worse outcome 
      for patients with an ABC DLBCL and a shorter overall survival for patients with 
      IgM+ tumor (P=0.21 and 0.014, respectively). Finally, fluorescence in situ 
      hybridization (FISH) analysis revealed a striking asymmetric pattern, as the IGHM 
      gene is conserved only on the productive IGH allele in most IgM+ tumors. Taken 
      together, these data indicate that the isotype of the BCR is a reliable indicator 
      for the GCB and ABC subtypes in DLBCL, and suggest that the conservation of an 
      IgM is required for ABC DLBCL lymphomagenesis to occur.
FAU - Ruminy, P
AU  - Ruminy P
AD  - INSERM U918, Laboratoire de genetique oncologique, Genetique et clinique des 
      proliferations lymphoides, IFRMP23, Centre Henri Becquerel, Rouen, France. 
      phirum@rouen.fnclcc.fr
FAU - Etancelin, P
AU  - Etancelin P
FAU - Couronne, L
AU  - Couronne L
FAU - Parmentier, F
AU  - Parmentier F
FAU - Rainville, V
AU  - Rainville V
FAU - Mareschal, S
AU  - Mareschal S
FAU - Bohers, E
AU  - Bohers E
FAU - Burgot, C
AU  - Burgot C
FAU - Cornic, M
AU  - Cornic M
FAU - Bertrand, P
AU  - Bertrand P
FAU - Lenormand, B
AU  - Lenormand B
FAU - Picquenot, J-M
AU  - Picquenot JM
FAU - Jardin, F
AU  - Jardin F
FAU - Tilly, H
AU  - Tilly H
FAU - Bastard, C
AU  - Bastard C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110114
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin M)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - B-Lymphocytes/*pathology
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Germinal Center/*pathology
MH  - Humans
MH  - Immunoenzyme Techniques
MH  - Immunoglobulin Heavy Chains/genetics
MH  - Immunoglobulin M/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, Large B-Cell, Diffuse/*classification/*genetics
MH  - Male
MH  - Middle Aged
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA, Messenger/genetics
MH  - Receptors, Antigen, B-Cell/*genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Survival Rate
EDAT- 2011/01/15 06:00
MHDA- 2011/06/10 06:00
CRDT- 2011/01/15 06:00
PHST- 2011/01/15 06:00 [entrez]
PHST- 2011/01/15 06:00 [pubmed]
PHST- 2011/06/10 06:00 [medline]
AID - leu2010302 [pii]
AID - 10.1038/leu.2010.302 [doi]
PST - ppublish
SO  - Leukemia. 2011 Apr;25(4):681-8. doi: 10.1038/leu.2010.302. Epub 2011 Jan 14.