PMID- 21234422
OWN - NLM
STAT- MEDLINE
DCOM- 20110715
LR  - 20211020
IS  - 1110-7251 (Electronic)
IS  - 1110-7243 (Print)
IS  - 1110-7243 (Linking)
VI  - 2011
DP  - 2011
TI  - The selection and use of sorghum (Sorghum propinquum) bacterial artificial 
      chromosomes as cytogenetic FISH probes for maize (Zea mays L.).
PG  - 386862
LID - 10.1155/2011/386862 [doi]
LID - 386862
AB  - The integration of genetic and physical maps of maize is progressing rapidly, but 
      the cytogenetic maps lag behind, with the exception of the pachytene fluorescence 
      in situ hybridization (FISH) maps of maize chromosome 9. We sought to produce 
      integrated FISH maps of other maize chromosomes using Core Bin Marker loci. 
      Because these 1 Kb restriction fragment length polymorphism (RFLP) probes are 
      below the FISH detection limit, we used BACs from sorghum, a small-genome 
      relative of maize, as surrogate clones for FISH mapping. We sequenced 151 maize 
      RFLP probes and compared in silico BAC selection methods to that of library 
      filter hybridization and found the latter to be the best. BAC library screening, 
      clone verification, and single-clone selection criteria are presented along with 
      an example of transgenomic BAC FISH mapping. This strategy has been used to 
      facilitate the integration of RFLP and FISH maps in other large-genome species.
FAU - Figueroa, Debbie M
AU  - Figueroa DM
AD  - Department of Biological Science, The Florida State University, Tallahassee, FL 
      32306-4370, USA.
FAU - Davis, James D
AU  - Davis JD
FAU - Strobel, Cornelia
AU  - Strobel C
FAU - Conejo, Maria S
AU  - Conejo MS
FAU - Beckham, Katherine D
AU  - Beckham KD
FAU - Ring, Brian C
AU  - Ring BC
FAU - Bass, Hank W
AU  - Bass HW
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20101222
PL  - United States
TA  - J Biomed Biotechnol
JT  - Journal of biomedicine & biotechnology
JID - 101135740
RN  - 0 (DNA Probes)
RN  - 0 (Genetic Markers)
RN  - EN464416SI (Ethidium)
SB  - IM
MH  - Blotting, Southern
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - DNA Probes/*metabolism
MH  - Electrophoresis, Agar Gel
MH  - Ethidium/metabolism
MH  - Genetic Loci/genetics
MH  - Genetic Markers
MH  - Genome, Plant/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Physical Chromosome Mapping
MH  - Plants, Genetically Modified
MH  - Polymorphism, Restriction Fragment Length/genetics
MH  - Reproducibility of Results
MH  - Sequence Analysis, DNA
MH  - Sorghum/*genetics
MH  - Zea mays/*genetics
PMC - PMC3014715
EDAT- 2011/01/15 06:00
MHDA- 2011/07/16 06:00
PMCR- 2010/12/22
CRDT- 2011/01/15 06:00
PHST- 2010/08/13 00:00 [received]
PHST- 2010/11/02 00:00 [accepted]
PHST- 2011/01/15 06:00 [entrez]
PHST- 2011/01/15 06:00 [pubmed]
PHST- 2011/07/16 06:00 [medline]
PHST- 2010/12/22 00:00 [pmc-release]
AID - 10.1155/2011/386862 [doi]
PST - ppublish
SO  - J Biomed Biotechnol. 2011;2011:386862. doi: 10.1155/2011/386862. Epub 2010 Dec 
      22.