PMID- 21235753
OWN - NLM
STAT- MEDLINE
DCOM- 20110419
LR  - 20211020
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 12
DP  - 2011 Jan 14
TI  - Divergent responses to peptidoglycans derived from different E. coli serotypes 
      influence inflammatory outcome in trout, Oncorhynchus mykiss, macrophages.
PG  - 34
LID - 10.1186/1471-2164-12-34 [doi]
AB  - BACKGROUND: Pathogen-associated molecular patterns (PAMPs) are structural 
      components of pathogens such as lipopolysaccharide (LPS) and peptidoglycan (PGN) 
      from bacterial cell walls. PAMP-recognition by the host results in an induction 
      of defence-related genes and often the generation of an inflammatory response. We 
      evaluated both the transcriptomic and inflammatory response in trout (O. mykiss) 
      macrophages in primary cell culture stimulated with DAP-PGN (DAP; 
      meso-diaminopimelic acid, PGN; peptidoglycan) from two strains of Escherichia 
      coli (PGN-K12 and PGN-O111:B4) over time. RESULTS: Transcript profiling was 
      assessed using function-targeted cDNA microarray hybridisation (n = 36) and 
      results show differential responses to both PGNs that are both time and treatment 
      dependent. Wild type E. coli (K12) generated an increase in transcript 
      number/diversity over time whereas PGN-O111:B4 stimulation resulted in a more 
      specific and intense response. In line with this, Gene Ontology analysis (GO) 
      highlights a specific transcriptomic remodelling for PGN-O111:B4 whereas results 
      obtained for PGN-K12 show a high similarity to a generalised inflammatory priming 
      response where multiple functional classes are related to ribosome biogenesis or 
      cellular metabolism. Prostaglandin release was induced by both PGNs and 
      macrophages were significantly more sensitive to PGN-O111:B4 as suggested from 
      microarray data. CONCLUSION: Responses at the level of the transcriptome and the 
      inflammatory outcome (prostaglandin synthesis) highlight the different 
      sensitivity of the macrophage to slight differences (serotype) in peptidoglycan 
      structure. Such divergent responses are likely to involve differential receptor 
      sensitivity to ligands or indeed different receptor types. Such changes in 
      biological response will likely reflect upon pathogenicity of certain serotypes 
      and the development of disease.
FAU - Boltana, Sebastian
AU  - Boltana S
AD  - Institute of Biotechnology and Biomedicine, Universitat Autonoma de Barcelona, 
      08193 Barcelona, Spain.
FAU - Reyes-Lopez, Felipe
AU  - Reyes-Lopez F
FAU - Morera, Davinia
AU  - Morera D
FAU - Goetz, Frederick
AU  - Goetz F
FAU - MacKenzie, Simon A
AU  - MacKenzie SA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110114
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Peptidoglycan)
RN  - 583-93-7 (Diaminopimelic Acid)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Diaminopimelic Acid/metabolism/*pharmacology
MH  - Escherichia coli/*metabolism
MH  - Macrophages/*drug effects/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Oncorhynchus mykiss/*immunology
MH  - Peptidoglycan/metabolism/*pharmacology
MH  - Polymerase Chain Reaction
PMC - PMC3087353
EDAT- 2011/01/18 06:00
MHDA- 2011/04/20 06:00
PMCR- 2011/01/14
CRDT- 2011/01/18 06:00
PHST- 2010/06/16 00:00 [received]
PHST- 2011/01/14 00:00 [accepted]
PHST- 2011/01/18 06:00 [entrez]
PHST- 2011/01/18 06:00 [pubmed]
PHST- 2011/04/20 06:00 [medline]
PHST- 2011/01/14 00:00 [pmc-release]
AID - 1471-2164-12-34 [pii]
AID - 10.1186/1471-2164-12-34 [doi]
PST - epublish
SO  - BMC Genomics. 2011 Jan 14;12:34. doi: 10.1186/1471-2164-12-34.