PMID- 21236710
OWN - NLM
STAT- MEDLINE
DCOM- 20110628
LR  - 20211203
IS  - 1528-395X (Electronic)
IS  - 1079-2104 (Linking)
VI  - 111
IP  - 3
DP  - 2011 Mar
TI  - Transforming growth factor beta1 down-regulates Runx-2 and alkaline phosphatase 
      activity of human dental pulp cells via ALK5/Smad2/3 signaling.
PG  - 394-400
LID - 10.1016/j.tripleo.2010.09.079 [doi]
AB  - OBJECTIVE: Transforming growth factor beta1 (TGF-beta1) plays a role in repair and 
      dentinogenesis in dental pulp. The purpose of this study was to study how TGF-beta1 
      affects 2 differentiation markers, Runt-related transcription factor 2 (Runx-2) 
      and ALP, in dental pulp cells. STUDY DESIGN: Primary-cultured human dental pulp 
      cells were treated with TGF-beta1 with or without pretreatment and coincubation with 
      1,4-diamino-2,3-dicyano-1,4-bis(o-aminophenylmercapto)butadiene (U0126, a 
      mitogen-induced extracellular kinase (MEK)/extracellular signal-regulated kinase 
      (ERK) inhibitor), Noggin (a bone morphogenetic protein inhibitor), or 
      4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl-1H-imidazol-2-yl)-benzamide hydrate 
      (SB431542, an activin receptor-like kinase (ALK) 5/Smad2/3 inhibitor). The 
      differentiation status of pulp cells was evaluated by ALP staining and 
      quantitative ALP activity assay. Changes in ALP and Runx-2 mRNA expression were 
      determined by reverse-transcription polymerase chain reaction. RESULTS: Cells 
      under the treatment of TGF-beta1 (5 and 10 ng/mL) showed a decrease in ALP activity 
      and gene expression of ALP and Runx-2. Pretreatment by U0126 and Noggin was not 
      effective to prevent the TGF-beta1-induced decline of ALP activity. Interestingly, 
      SB431542 prevented the TGF-beta1-induced decline of ALP activity and ALP and Runx-2 
      gene expression. CONCLUSION: TGF-beta1 down-regulates Runx-2 and ALP in human dental 
      pulp cells via ALK5/Smad2/3 signaling. These events may play important roles at 
      specific stages of pulpal repair and dentinogenesis.
CI  - Copyright (c) 2011 Mosby, Inc. All rights reserved.
FAU - Lin, Po-Shuen
AU  - Lin PS
AD  - Department of Dentistry/School of Dentistry, National Taiwan University Hospital 
      and National Taiwan University College of Medicine, Taipei, Taiwan.
FAU - Chang, Mei-Chi
AU  - Chang MC
FAU - Chan, Chiu-Po
AU  - Chan CP
FAU - Lee, Sheng-Yang
AU  - Lee SY
FAU - Lee, Jang-Jaer
AU  - Lee JJ
FAU - Tsai, Yi-Ling
AU  - Tsai YL
FAU - Tseng, Hui-Chun
AU  - Tseng HC
FAU - Tai, Tseng-Fang
AU  - Tai TF
FAU - Lin, Hsueh-Jen
AU  - Lin HJ
FAU - Jeng, Jiiang-Huei
AU  - Jeng JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110113
PL  - United States
TA  - Oral Surg Oral Med Oral Pathol Oral Radiol Endod
JT  - Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics
JID - 9508562
RN  - 0 (4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamide)
RN  - 0 (Benzamides)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Butadienes)
RN  - 0 (Carrier Proteins)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Dioxoles)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Nitriles)
RN  - 0 (RUNX2 protein, human)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (SMAD2 protein, human)
RN  - 0 (SMAD3 protein, human)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad3 Protein)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (U 0126)
RN  - 148294-77-3 (noggin protein)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type I)
RN  - EC 2.7.11.30 (TGFBR1 protein, human)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/antagonists & inhibitors/*metabolism
MH  - Benzamides/pharmacology
MH  - Bone Morphogenetic Proteins/antagonists & inhibitors
MH  - Butadienes/pharmacology
MH  - Carrier Proteins/pharmacology
MH  - Cells, Cultured
MH  - Core Binding Factor Alpha 1 Subunit/antagonists & inhibitors/*metabolism
MH  - Dental Pulp/cytology/enzymology/*metabolism
MH  - Dioxoles/pharmacology
MH  - Down-Regulation/*physiology
MH  - Enzyme Inhibitors/pharmacology
MH  - Humans
MH  - Mitogen-Activated Protein Kinase Kinases/antagonists & inhibitors
MH  - Nitriles/pharmacology
MH  - Protein Serine-Threonine Kinases/antagonists & inhibitors/*metabolism
MH  - Receptor, Transforming Growth Factor-beta Type I
MH  - Receptors, Transforming Growth Factor beta/antagonists & inhibitors/*metabolism
MH  - Signal Transduction/drug effects/*physiology
MH  - Smad2 Protein/antagonists & inhibitors/*metabolism
MH  - Smad3 Protein/antagonists & inhibitors/*metabolism
MH  - Transforming Growth Factor beta1/*metabolism/pharmacology
EDAT- 2011/01/18 06:00
MHDA- 2011/06/29 06:00
CRDT- 2011/01/18 06:00
PHST- 2010/08/03 00:00 [received]
PHST- 2010/08/24 00:00 [revised]
PHST- 2010/09/29 00:00 [accepted]
PHST- 2011/01/18 06:00 [entrez]
PHST- 2011/01/18 06:00 [pubmed]
PHST- 2011/06/29 06:00 [medline]
AID - S1079-2104(10)00798-5 [pii]
AID - 10.1016/j.tripleo.2010.09.079 [doi]
PST - ppublish
SO  - Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Mar;111(3):394-400. doi: 
      10.1016/j.tripleo.2010.09.079. Epub 2011 Jan 13.