PMID- 21237132
OWN - NLM
STAT- MEDLINE
DCOM- 20110406
LR  - 20181201
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 405
IP  - 2
DP  - 2011 Feb 11
TI  - Epiregulin-dependent amphiregulin expression and ERBB2 signaling are involved in 
      luteinizing hormone-induced paracrine signaling pathways in mouse ovary.
PG  - 319-24
LID - 10.1016/j.bbrc.2011.01.039 [doi]
AB  - Sustained EGF receptor (EGFR) phosphorylation by de novo synthesis of EGFR 
      ligands plays an essential role in mediating luteinizing hormone (LH)-induced 
      ovulation process in the preovulatory follicles (POFs). In the present study, the 
      effect of epiregulin (EREG) on oocyte maturation and ovulation was investigated 
      using Ereg knockout (Ereg-/-) mice congenic on a C57BL/6 background. Rate of 
      spontaneous oocyte meiotic resumption of denuded oocytes (DOs) or cumulus 
      cell-oocyte complexes (COCs) in vitro is similar between wild-type and Ereg-/- 
      mice. However, gonadotropin-induced meiotic resumption in vivo is attenuated, and 
      the number of COCs with expanded cumulus matrix and superovulated eggs 
      dramatically decrease in Ereg-/- mice. Nonetheless, the number of eggs ovulated 
      during normal estrus cycles and litter sizes in Ereg-/- mice are comparable to 
      those of wild-type littermates. In contrast to other EGFR ligands, induction of 
      amphiregulin (Areg) mRNA is severely reduced in ovaries collected from Ereg-/- 
      mice either after human chorionic gonadotropin (hCG) treatment in immature mice 
      or LH surge in adults. Gonadotropin-induced EGFR and ERBB2 phosphorylation in 
      ovaries is attenuated in immature Ereg-/- mice, and MAPK3/1 phosphorylation and 
      prostaglandin synthase 2 (PTGS2) protein levels are reduced. This attenuation, 
      however, is no longer detectable in adult Ereg-/- mice after LH surge. This study 
      implicates that EREG mediates signals downstream of Areg mRNA expression and that 
      EGFR-ERBB2 signals contributes to regulation of ovulation process.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Kim, Kyoungmi
AU  - Kim K
AD  - Department of Life Science and Pharmaceutical Science, Ewha Womans University, 
      Seoul, South Korea.
FAU - Lee, Hyunji
AU  - Lee H
FAU - Threadgill, David W
AU  - Threadgill DW
FAU - Lee, Daekee
AU  - Lee D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110113
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Areg protein, mouse)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (EREG protein, human)
RN  - 0 (Epiregulin)
RN  - 0 (Ereg protein, mouse)
RN  - 0 (Glycoproteins)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (RNA, Messenger)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - EC 2.7.10.1 (EGFR protein, mouse)
RN  - EC 2.7.10.1 (ErbB Receptors)
RN  - EC 2.7.10.1 (Erbb2 protein, mouse)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Amphiregulin
MH  - Animals
MH  - EGF Family of Proteins
MH  - Epidermal Growth Factor/genetics/*metabolism
MH  - Epiregulin
MH  - ErbB Receptors/metabolism
MH  - Female
MH  - Glycoproteins/*biosynthesis/genetics
MH  - Intercellular Signaling Peptides and Proteins/*biosynthesis/genetics
MH  - Luteinizing Hormone/*metabolism
MH  - Meiosis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Ovary/cytology/metabolism/*physiology
MH  - *Ovulation
MH  - Phosphorylation
MH  - RNA, Messenger/biosynthesis
MH  - Receptor, ErbB-2/*metabolism
MH  - Signal Transduction
EDAT- 2011/01/18 06:00
MHDA- 2011/04/07 06:00
CRDT- 2011/01/18 06:00
PHST- 2011/01/05 00:00 [received]
PHST- 2011/01/08 00:00 [accepted]
PHST- 2011/01/18 06:00 [entrez]
PHST- 2011/01/18 06:00 [pubmed]
PHST- 2011/04/07 06:00 [medline]
AID - S0006-291X(11)00058-1 [pii]
AID - 10.1016/j.bbrc.2011.01.039 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Feb 11;405(2):319-24. doi: 
      10.1016/j.bbrc.2011.01.039. Epub 2011 Jan 13.