PMID- 2123886
OWN - NLM
STAT- MEDLINE
DCOM- 19910124
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 28
IP  - 11
DP  - 1990 Nov
TI  - Enhancement of anti-influenza A virus cytotoxicity following influenza A virus 
      vaccination in older, chronically ill adults.
PG  - 2539-50
AB  - We studied anti-influenza cytotoxicity by bulk peripheral blood mononuclear 
      leukocyte (PBL) cultures derived from older, chronically ill volunteers 
      undergoing vaccination. Vaccinees received either cold-recombinant, 
      live-attenuated influenza A/Korea/1/82 (H3N2) virus intranasally or inactivated 
      monovalent influenza A/Taiwan/1/86 (H1N1) subvirion vaccine intramuscularly. PBL 
      were collected pre- and postvaccination and in vitro stimulated by autologous PBL 
      infected with influenza A virus homologous and heterosubtypic to the respective 
      vaccine strain. Cytotoxicity was measured against influenza A virus-infected 
      autologous and human leukocyte antigen (HLA)-mismatched PBL targets infected with 
      influenza A virus homologous or heterosubtypic to the vaccine virus strain. 
      Vaccinees infected with the live-attenuated virus developed significant rises in 
      mean anti-influenza, HLA-restricted cytotoxicity that was cross-reactive against 
      influenza A viruses homologous and heterosubtypic to the vaccine virus. The 
      enhanced cross-reactive cytotoxicity was inducible postvaccination by in vitro 
      stimulation with autologous PBL infected with the homologous influenza A (H3N2) 
      virus and with influenza A (H1N1) virus. In contrast, after vaccination with 
      inactivated monovalent subvirion vaccine, volunteers developed significant 
      increases in mean anti-influenza, HLA-restricted cytotoxicity only against 
      autologous PBL infected with homologous influenza A (H1N1) virus. Increased 
      cytotoxicity occurred only after in vitro stimulation with autologous cells 
      infected with homologous influenza A (H1N1) virus. Mean gamma interferon levels 
      in supernatant fluids of influenza A virus-stimulated effector PBL did not 
      increase postvaccination, despite increased levels of anti-influenza cytotoxicity 
      displayed by the effector cells. We conclude that the live-attenuated influenza A 
      virus infection induced a broader range of enhanced anti-influenza cytotoxicity 
      than did the inactivated subvirion vaccine.
FAU - Gorse, G J
AU  - Gorse GJ
AD  - Huntington Veterans Affairs Medical Center, West Virginia.
FAU - Belshe, R B
AU  - Belshe RB
LA  - eng
GR  - N01-AI-05051/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (HLA Antigens)
RN  - 0 (Influenza Vaccines)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Vaccines, Inactivated)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Aged
MH  - Cross Reactions
MH  - *Cytotoxicity, Immunologic
MH  - HLA Antigens
MH  - Humans
MH  - In Vitro Techniques
MH  - Influenza A virus/classification/*immunology
MH  - Influenza Vaccines/*pharmacology
MH  - Interferon-gamma/biosynthesis
MH  - Middle Aged
MH  - Vaccines, Attenuated/pharmacology
MH  - Vaccines, Inactivated/pharmacology
PMC - PMC268221
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
PMCR- 1990/11/01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
PHST- 1990/11/01 00:00 [pmc-release]
AID - 10.1128/jcm.28.11.2539-2550.1990 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1990 Nov;28(11):2539-50. doi: 10.1128/jcm.28.11.2539-2550.1990.