PMID- 21239527
OWN - NLM
STAT- MEDLINE
DCOM- 20110707
LR  - 20191210
IS  - 1741-7899 (Electronic)
IS  - 1470-1626 (Linking)
VI  - 141
IP  - 4
DP  - 2011 Apr
TI  - Effect of epidermal growth factor-like peptides on pig cumulus cell expansion, 
      oocyte maturation, and acquisition of developmental competence in vitro: 
      comparison with gonadotropins.
PG  - 425-35
LID - 10.1530/REP-10-0418 [doi]
AB  - The aim of this work was to assess the FSH-stimulated expression of epidermal 
      growth factor (EGF)-like peptides in cultured cumulus-oocyte complexes (COCs) and 
      to find out the effect of the peptides on cumulus expansion, oocyte maturation, 
      and acquisition of developmental competence in vitro. FSH promptly stimulated 
      expression of amphiregulin (AREG) and epiregulin (EREG), but not betacellulin 
      (BTC) in the cultured COCs. Expression of AREG and EREG reached maximum at 2 or 4 
      h after FSH addition respectively. FSH also significantly stimulated expression 
      of expansion-related genes (PTGS2, TNFAIP6, and HAS2) in the COCs at 4 and 8 h of 
      culture, with a significant decrease at 20 h of culture. Both AREG and EREG also 
      increased expression of the expansion-related genes; however, the relative 
      abundance of mRNA for each gene was much lower than in the FSH-stimulated COCs. 
      In contrast to FSH, AREG and EREG neither stimulated expression of CYP11A1 in the 
      COCs nor an increase in progesterone production by cumulus cells. AREG and EREG 
      stimulated maturation of oocytes and expansion of cumulus cells, although the 
      percentage of oocytes that had reached metaphase II was significantly lower when 
      compared to FSH-induced maturation. Nevertheless, significantly more oocytes 
      stimulated with AREG and/or EREG developed to blastocyst stage after 
      parthenogenetic activation when compared to oocytes stimulated with FSH alone or 
      combinations of FSH/LH or pregnant mares serum gonadotrophin/human chorionic 
      gonadotrophin. We conclude that EGF-like peptides do not mimic all effects of FSH 
      on the cultured COCs; nevertheless, they yield oocytes with superior 
      developmental competence.
FAU - Prochazka, Radek
AU  - Prochazka R
AD  - Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech 
      Republic, Rumburska 89, 277 21 Libechov, Czech Republic. prochazka@iapg.cas.cz
FAU - Petlach, Michal
AU  - Petlach M
FAU - Nagyova, Eva
AU  - Nagyova E
FAU - Nemcova, Lucie
AU  - Nemcova L
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110114
PL  - England
TA  - Reproduction
JT  - Reproduction (Cambridge, England)
JID - 100966036
RN  - 0 (Gonadotropins)
RN  - 0 (Peptide Fragments)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects/genetics
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cumulus Cells/*drug effects/metabolism/physiology
MH  - Embryo Culture Techniques
MH  - Embryonic Development/drug effects/genetics
MH  - Epidermal Growth Factor/chemistry/pharmacology
MH  - Female
MH  - Follicle Stimulating Hormone/pharmacology
MH  - Gene Expression Profiling
MH  - Gonadotropins/*pharmacology
MH  - Oocytes/*drug effects/metabolism/physiology
MH  - Oogenesis/drug effects/genetics
MH  - Parthenogenesis/drug effects/genetics/physiology
MH  - Peptide Fragments/chemistry/*pharmacology
MH  - *Swine/genetics/metabolism/physiology
EDAT- 2011/01/18 06:00
MHDA- 2011/07/08 06:00
CRDT- 2011/01/18 06:00
PHST- 2011/01/18 06:00 [entrez]
PHST- 2011/01/18 06:00 [pubmed]
PHST- 2011/07/08 06:00 [medline]
AID - REP-10-0418 [pii]
AID - 10.1530/REP-10-0418 [doi]
PST - ppublish
SO  - Reproduction. 2011 Apr;141(4):425-35. doi: 10.1530/REP-10-0418. Epub 2011 Jan 14.