PMID- 21241770 OWN - NLM STAT- MEDLINE DCOM- 20110926 LR - 20151119 IS - 1872-8057 (Electronic) IS - 0303-7207 (Linking) VI - 337 IP - 1-2 DP - 2011 Apr 30 TI - Perfluorooctanoic acid-induced inhibition of placental prolactin-family hormone and fetal growth retardation in mice. PG - 7-15 LID - 10.1016/j.mce.2011.01.009 [doi] AB - Perfluorooctanoic acid (PFOA) is a persistent pollutant worldwide and even found in human cord blood and breast milk. Some animal studies have reported that PFOA causes developmental toxicity such as fetal weight loss, but the mechanism is still unclear. This study focused on developmental toxicity of PFOA, particularly impacts of PFOA on placental endocrine function such as placental prolactin (PRL)-family hormone gene expression and fetal growth in mouse. Time-mated CD-1 mice were dosed by gavage with 0, 2, 10 and 25 mg/kg B.W/day of PFOA (n-10) dissolved with de-ionized water from gestational day (GD) 11-16. During treatment, body weight of each pregnant mouse was measured daily. On day 16, caesarean sections were performed and developmental data were observed. Three placentas from three different pregnant mice were assigned to each of the following experiments. The mRNA levels of mouse placental lactogen (mPL)-II, prolactin like protein (mPLP)-E, -F and Pit-1alpha and beta isotype mRNAs, a transacting factor of mPLs and mPLPs genes, were analyzed using northern blot, in situ hybridization and RT-PCR, respectively. Maternal body weight gain was significantly declined from GD 13 in the PFOA treated groups compared to control. Developmental data such as fetal and placental weights were significantly decreased in accordance with PFOA dosage. Number of dead fetuses and post-implantation losses were significantly increased in the PFOA-exposed groups. In addition, placental efficiency (fetal weight/placental weight) was significantly reduced in PFOA treated groups in accordance with PFOA dosage. Histopathologic changes were observed in placenta. Dose dependent necrotic changes were observed in both 10 mg and 25 mg PFOA treated groups. Cell frequency of glycogen trophoblast cell and parietal trophoblast giant cell were decreased dose dependently in the junctional zone. In the labyrinth zone, sinusoidal trophoblast giant cell frequency was decreased in the 25 mg PFOA treated group. Also, morphological change such as crushed nuclear (atrophy) of trophoblast cells was observed in 25 mg PFOA treated group. Finally, mRNA levels of the mPL-II, mPLP-E, -F and Pit-1alpha and beta were significantly reduced in the PFOA treated groups dose dependently. In addition, the changing pattern between mPL-II, mPLP-E, -F mRNA levels and fetal body weight showed positive relationship. In conclusion, the inhibitory effects of PFOA on the placental prolactin-family hormone genes expression may be secondary effects to insufficient trophoblast cell type differentiation and/or increased trophoblast cell necrosis. The impacts of PFOA on placental development and endocrine function reduced the placental efficiency and partly contributed to the fetal growth retardation in the mouse. CI - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved. FAU - Suh, Chun Hui AU - Suh CH AD - Institute of Environmental and Occupational Medicine & Department of Occupational and Environmental Medicine, College of Medicine, Inje University, South Korea. FAU - Cho, Nam Kyoo AU - Cho NK FAU - Lee, Chae Kwan AU - Lee CK FAU - Lee, Chang Hee AU - Lee CH FAU - Kim, Dae Hwan AU - Kim DH FAU - Kim, Jeong Ho AU - Kim JH FAU - Son, Byung Chul AU - Son BC FAU - Lee, Jong Tae AU - Lee JT LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20110115 PL - Ireland TA - Mol Cell Endocrinol JT - Molecular and cellular endocrinology JID - 7500844 RN - 0 (Caprylates) RN - 0 (Cytokines) RN - 0 (Fluorocarbons) RN - 0 (Pit1 protein, mouse) RN - 0 (Placental Hormones) RN - 0 (Pregnancy Proteins) RN - 0 (Prlpe protein, mouse) RN - 0 (Prlpf protein, mouse) RN - 0 (Transcription Factor Pit-1) RN - 9035-54-5 (Placental Lactogen) RN - 947VD76D3L (perfluorooctanoic acid) SB - IM MH - Animals MH - Body Weight/drug effects MH - Caprylates/*adverse effects MH - Cell Count MH - Cytokines/genetics/metabolism MH - Female MH - Fetal Death/chemically induced MH - Fetal Growth Retardation/*chemically induced MH - Fetal Weight/drug effects MH - Fluorocarbons/*adverse effects MH - Mice MH - Placenta/drug effects/pathology MH - Placental Hormones/*antagonists & inhibitors MH - Placental Lactogen/genetics/metabolism MH - Pregnancy MH - Pregnancy Proteins/genetics/metabolism MH - Transcription Factor Pit-1/genetics/metabolism MH - Transcription, Genetic/drug effects MH - Trophoblasts/cytology/drug effects EDAT- 2011/01/19 06:00 MHDA- 2011/09/29 06:00 CRDT- 2011/01/19 06:00 PHST- 2010/07/13 00:00 [received] PHST- 2011/01/06 00:00 [revised] PHST- 2011/01/10 00:00 [accepted] PHST- 2011/01/19 06:00 [entrez] PHST- 2011/01/19 06:00 [pubmed] PHST- 2011/09/29 06:00 [medline] AID - S0303-7207(11)00031-1 [pii] AID - 10.1016/j.mce.2011.01.009 [doi] PST - ppublish SO - Mol Cell Endocrinol. 2011 Apr 30;337(1-2):7-15. doi: 10.1016/j.mce.2011.01.009. Epub 2011 Jan 15.