PMID- 21242807
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20131121
IS  - 1533-4023 (Electronic)
IS  - 0160-2446 (Linking)
VI  - 57
IP  - 4
DP  - 2011 Apr
TI  - Differential modulation of immunostimulant-triggered NO production by endoplasmic 
      reticulum stress inducers in vascular smooth muscle cells.
PG  - 434-8
LID - 10.1097/FJC.0b013e31820d9486 [doi]
AB  - We investigated the effects of endoplasmic reticulum (ER) stress inducers 
      thapsigargin (TG) and tunicamycin (Tm) on immunostimulant 
      lipopolysaccharide/interferon (LPS/IFN)-induced expression of isoform of nitric 
      oxide synthase (iNOS) and nitric oxide (NO) production in vascular smooth muscle 
      cells. LPS/IFN-induced iNOS mRNA expression was markedly enhanced by TG, whereas 
      iNOS mRNA expression was strongly attenuated by Tm. Similarly, production of iNOS 
      protein was markedly upregulated by TG but virtually eliminated by Tm. 
      LPS/IFN-induced guanosine triphosphate cyclohydrolase I mRNA expression was 
      slightly reduced by TG and markedly inhibited by Tm. Similarly, LPS/IFN-mediated 
      induction of cellular biopterin was modestly reduced by TG and markedly inhibited 
      by Tm. TG modestly enhanced LPS/IFN-induced activation of NF-kappaB, whereas Tm had 
      no effect on it. Cellular respiration was reduced by TG and Tm in a 
      concentration-dependent manner, which was confirmed by apoptosis assay. Thus, TG 
      and Tm-induced ER stress and differently modulated NO production through 
      alterations in iNOS expression and activity independently of NF-kappaB activation and 
      caused a similar degree of ER stress-induced apoptosis.
FAU - Ohta, Satoshi
AU  - Ohta S
AD  - Department of Endocrinology and Metabolism, Dokkyo University School of Medicine, 
      Mibu, Tochigi, Japan.
FAU - Hattori, Yoshiyuki
AU  - Hattori Y
FAU - Nakanishi, Nobuo
AU  - Nakanishi N
FAU - Sugimoto, Hiroyuki
AU  - Sugimoto H
FAU - Kasai, Kikuo
AU  - Kasai K
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Cardiovasc Pharmacol
JT  - Journal of cardiovascular pharmacology
JID - 7902492
RN  - 0 (Lipopolysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 11089-65-9 (Tunicamycin)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 67526-95-8 (Thapsigargin)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 3.5.4.16 (GTP Cyclohydrolase)
SB  - IM
MH  - Animals
MH  - Cell Respiration/drug effects
MH  - Dose-Response Relationship, Drug
MH  - Endoplasmic Reticulum/*drug effects/metabolism
MH  - GTP Cyclohydrolase/drug effects/genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Interferon-gamma/pharmacology
MH  - Lipopolysaccharides/pharmacology
MH  - Male
MH  - Muscle, Smooth, Vascular/cytology/drug effects/metabolism
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type II/drug effects/genetics/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Thapsigargin/administration & dosage/*pharmacology
MH  - Tunicamycin/administration & dosage/*pharmacology
EDAT- 2011/01/19 06:00
MHDA- 2011/07/30 06:00
CRDT- 2011/01/19 06:00
PHST- 2011/01/19 06:00 [entrez]
PHST- 2011/01/19 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - 10.1097/FJC.0b013e31820d9486 [doi]
PST - ppublish
SO  - J Cardiovasc Pharmacol. 2011 Apr;57(4):434-8. doi: 10.1097/FJC.0b013e31820d9486.