PMID- 21242884
OWN - NLM
STAT- MEDLINE
DCOM- 20110414
LR  - 20171213
IS  - 1534-6080 (Electronic)
IS  - 0041-1337 (Linking)
VI  - 91
IP  - 5
DP  - 2011 Mar 15
TI  - Tissue specificity of cross-reactive allogeneic responses by EBV EBNA3A-specific 
      memory T cells.
PG  - 494-500
LID - 10.1097/TP.0b013e318207944c [doi]
AB  - BACKGROUND: The crossreactivity of Epstein-Barr virus (EBV Epstein-Barr virus 
      nuclear antigen 3A [EBNA3A])-specific CD8 T cells against allogeneic human 
      leukocyte antigen (HLA)-B*44:02 has been shown to be dependent on presentation of 
      self-peptide EEYLQAFTY by the target antigen. In this study, we report that 
      allogeneic HLA-B*44:02 proximal tubular epithelial cells (PTECs) and human 
      umbilical vein endothelial cells (HUVECs) are poor targets for EBV 
      EBNA3A-specific T cells. METHODS: The EEY peptide was exogenously loaded onto 
      HLA-B*44:02 and HLA-B*44:03-expressing PTECs and HUVECs. EEY-peptide-loaded, and 
      unloaded, PTECs and HUVECs were then incubated with serial dilutions of our 
      EBNA3A T-cell clone, in a cytotoxicity assay. RESULTS: Although 
      HLA-B*44:02-expressing PTECs were specifically lysed in proportion to the 
      effector/target ratio by the EBNA3A T-cell clone, without peptide loading, lysis 
      was greatly increased by exogenous EEY peptide loading (15% vs. 75%; P<0.0001). 
      HLA-B*44:02-expressing HUVECs were only lysed when loaded with exogenous EEY 
      peptide (0% vs. 64%; P<0.0001). Lack of HLA expression and lack of ABCD3 gene 
      expression were excluded as a cause for these results. PTECs and HUVECs were 
      specifically targeted by another alloreactive T-cell clone without exogenous 
      peptide loading, suggesting that the lack of recognition of HLA-B*44:02 
      epithelial and endothelial cells by the EBV EBNA3A T-cell clone was due to lack 
      of EEYLQAFTY peptide presentation. CONCLUSIONS: Tissue-specific (peptide 
      dependent) alloreactivity may have important implications for transplantation 
      monitoring and rejection.
FAU - D'Orsogna, Lloyd J A
AU  - D'Orsogna LJ
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Centre, Leiden, The Netherlands. l.j.a.d_orsogna@lumc.nl
FAU - Roelen, Dave L
AU  - Roelen DL
FAU - van der Meer-Prins, Ellen M W
AU  - van der Meer-Prins EM
FAU - van der Pol, Pieter
AU  - van der Pol P
FAU - Franke-van Dijk, Marry E
AU  - Franke-van Dijk ME
FAU - Eikmans, Michael
AU  - Eikmans M
FAU - Anholts, Jacqy
AU  - Anholts J
FAU - Rossjohn, Jamie
AU  - Rossjohn J
FAU - McCluskey, James
AU  - McCluskey J
FAU - Mulder, Arend
AU  - Mulder A
FAU - van Kooten, Cees
AU  - van Kooten C
FAU - Doxiadis, Ilias I N
AU  - Doxiadis II
FAU - Claas, Frans H J
AU  - Claas FH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (ABCD3 protein, human)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (EBNA-3A antigen)
RN  - 0 (Epstein-Barr Virus Nuclear Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B44 Antigen)
RN  - 0 (Peptides)
SB  - IM
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - CD8-Positive T-Lymphocytes/cytology/*immunology
MH  - Cells, Cultured
MH  - Cross Reactions/immunology
MH  - Endothelium, Vascular/cytology/immunology
MH  - Epstein-Barr Virus Nuclear Antigens/*immunology
MH  - HLA-B Antigens/metabolism
MH  - HLA-B44 Antigen
MH  - Herpesvirus 4, Human/*immunology
MH  - Humans
MH  - Kidney Tubules, Proximal/cytology/immunology
MH  - Organ Specificity/immunology
MH  - Peptides/metabolism
EDAT- 2011/01/19 06:00
MHDA- 2011/04/16 06:00
CRDT- 2011/01/19 06:00
PHST- 2011/01/19 06:00 [entrez]
PHST- 2011/01/19 06:00 [pubmed]
PHST- 2011/04/16 06:00 [medline]
AID - 10.1097/TP.0b013e318207944c [doi]
PST - ppublish
SO  - Transplantation. 2011 Mar 15;91(5):494-500. doi: 10.1097/TP.0b013e318207944c.