PMID- 21244501
OWN - NLM
STAT- MEDLINE
DCOM- 20111206
LR  - 20211020
IS  - 1600-0501 (Electronic)
IS  - 0905-7161 (Print)
IS  - 0905-7161 (Linking)
VI  - 22
IP  - 8
DP  - 2011 Aug
TI  - Osteoblast response to titanium surfaces functionalized with extracellular matrix 
      peptide biomimetics.
PG  - 865-72
LID - 10.1111/j.1600-0501.2010.02074.x [doi]
AB  - OBJECTIVE: Functionalizing surfaces with specific peptides may aid 
      osteointegration of orthopedic implants by favoring attachment of osteoprogenitor 
      cells and promoting osteoblastic differentiation. This study addressed the 
      hypothesis that implant surfaces functionalized with peptides targeting multiple 
      ligands will enhance osteoblast attachment and/or differentiation. To test this 
      hypothesis, we used titanium (Ti) surfaces coated with poly-l-lysine-grafted 
      polyethylene glycol (PLL-g-PEG) and functionalized with two peptides found in 
      extracellular matrix proteins, arginine-glycine-aspartic acid (RGD) and 
      lysine-arginine-serine-arginine (KRSR), which have been shown to increase 
      osteoblast attachment. KSSR, which does not promote osteoblast attachment, was 
      used as a control. MATERIALS AND METHODS: Sandblasted acid-etched titanium 
      surfaces were coated with PLL-g-PEG functionalized with varying combinations of 
      RGD and KRSR, as well as KSSR. Effects of these surfaces on osteoblasts were 
      assessed by measuring cell number, alkaline phosphatase-specific activity, and 
      levels of osteocalcin, transforming growth factor beta-1 (TGF-beta1), and PGE(2). 
      RESULTS: RGD increased cell number, but decreased markers for osteoblast 
      differentiation. KRSR alone had no effect on cell number, but decreased levels of 
      TGF-beta1 and PGE(2). KRSR and RGD/KRSR coatings inhibited osteoblast 
      differentiation vs. PLL-g-PEG. KSSR decreased cell number and increased 
      osteoblast differentiation, indicated by increased levels of osteocalcin and 
      PGE(2). CONCLUSIONS: The RGD and KRSR functionalized surfaces supported 
      attachment but did not enhance osteoblast differentiation, whereas KSSR increased 
      differentiation. RGD decreased this effect, suggesting that multifunctional 
      peptide surfaces can be designed that improve peri-implant healing by optimizing 
      attachment and proliferation as well as differentiation of osteoblasts, but 
      peptide combination, dose and presentation are critical variables.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Bell, B F
AU  - Bell BF
AD  - Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 
      Atlanta, GA 30332-0363, USA.
FAU - Schuler, M
AU  - Schuler M
FAU - Tosatti, S
AU  - Tosatti S
FAU - Textor, M
AU  - Textor M
FAU - Schwartz, Z
AU  - Schwartz Z
FAU - Boyan, B D
AU  - Boyan BD
LA  - eng
GR  - R01 AR052102/AR/NIAMS NIH HHS/United States
GR  - AR052102/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110118
PL  - Denmark
TA  - Clin Oral Implants Res
JT  - Clinical oral implants research
JID - 9105713
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Dental Materials)
RN  - 0 (Drug Carriers)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Oligopeptides)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (lysyl-arginyl-seryl-arginine)
RN  - 0 (polylysine-graft-(poly(ethylene glycol)))
RN  - 104982-03-8 (Osteocalcin)
RN  - 25104-18-1 (Polylysine)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 452VLY9402 (Serine)
RN  - 78VO7F77PN (arginyl-glycyl-aspartic acid)
RN  - 94ZLA3W45F (Arginine)
RN  - D1JT611TNE (Titanium)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - K3Z4F929H6 (Lysine)
RN  - K7Q1JQR04M (Dinoprostone)
MH  - Acid Etching, Dental/methods
MH  - Alkaline Phosphatase/analysis/drug effects
MH  - Arginine/pharmacology
MH  - Biomimetic Materials/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cell Line
MH  - Cell Proliferation/drug effects
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Dental Etching/methods
MH  - Dental Materials/*chemistry
MH  - Dinoprostone/analysis
MH  - Drug Carriers
MH  - Extracellular Matrix Proteins/*pharmacology
MH  - Humans
MH  - Lysine/pharmacology
MH  - Materials Testing
MH  - Oligopeptides/pharmacology
MH  - Osteoblasts/*drug effects
MH  - Osteocalcin/analysis/drug effects
MH  - Osteogenesis/drug effects
MH  - Polyethylene Glycols/chemistry
MH  - Polylysine/analogs & derivatives/chemistry
MH  - Serine/pharmacology
MH  - Titanium/*chemistry
MH  - Transforming Growth Factor beta1/analysis/drug effects
PMC - PMC4287399
MID - NIHMS640176
EDAT- 2011/01/20 06:00
MHDA- 2011/12/13 00:00
PMCR- 2015/01/08
CRDT- 2011/01/20 06:00
PHST- 2011/01/20 06:00 [entrez]
PHST- 2011/01/20 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2015/01/08 00:00 [pmc-release]
AID - 10.1111/j.1600-0501.2010.02074.x [doi]
PST - ppublish
SO  - Clin Oral Implants Res. 2011 Aug;22(8):865-72. doi: 
      10.1111/j.1600-0501.2010.02074.x. Epub 2011 Jan 18.