PMID- 2124580
OWN - NLM
STAT- MEDLINE
DCOM- 19910207
LR  - 20190510
IS  - 0021-8812 (Print)
IS  - 0021-8812 (Linking)
VI  - 68
IP  - 11
DP  - 1990 Nov
TI  - Active immunization of beef heifers against luteinizing hormone: II. Evaluation 
      of conjugation technique on antigenicity of LH.
PG  - 3747-54
AB  - This study evaluated the antigenicity of LH-ovalbumin complexes produced using 
      different conjugation techniques. Two homobifunctional cross-linkers, 
      glutaraldehyde (Glut) and carbodiimide (ECDI), were evaluated together with one 
      heterobifunctional reagent, m-maleimido-benzoyl N-hydroxysuccinimide ester (MBS). 
      Polyacrylamide gel electrophoresis and Western transfer techniques were used to 
      confirm conjugation of LH. Forty-four beef heifers were assigned randomly to 
      seven treatment groups. Two groups of heifers were immunized against 
      glutaraldehyde conjugates (Glut-I and Glut-II), two against MBS conjugates (MBS-I 
      and MBS-II) and one against a carbodiimide conjugate (ECDI). Control animals were 
      immunized against nonconjugated LH (LH-only) or ovalbumin alone (Oval). Heifers 
      received one primary injection of antigen followed by two boosters at a 3-wk 
      interval. The Glut conjugates induced the highest (P less than .05) LH antibody 
      activity (Glut-II, 18 +/- 4%; Glut-I, 14 +/- 4%). The ECDI (11 +/- 4%), and MBS-I 
      (11 +/- 2%) conjugates induced greater LH binding than MBS-II (4 +/- 1%), LH-only 
      (4 +/- 1%) or Oval (2 +/- 1%). Glutaraldehyde produced an LH-ovalbumin conjugate 
      of greater LH immunogenicity than either ECDI or the heterobifunctional reagent, 
      MBS.
FAU - Grieger, D M
AU  - Grieger DM
AD  - Washington State University, Pullman 99164-6332.
FAU - Reeves, J J
AU  - Reeves JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Succinimides)
RN  - 420-04-2 (Cyanamide)
RN  - 58626-38-3 (3-maleimidobenzoyl N-hydroxysuccinimide)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9006-59-1 (Ovalbumin)
RN  - T3C89M417N (Glutaral)
SB  - IM
MH  - Animals
MH  - Antibody Formation
MH  - Blotting, Western
MH  - Cattle/*immunology
MH  - *Cross-Linking Reagents
MH  - Cyanamide
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Glutaral
MH  - Immunization/*veterinary
MH  - Indicators and Reagents
MH  - Luteinizing Hormone/*immunology
MH  - Ovalbumin/*immunology
MH  - Random Allocation
MH  - Succinimides
EDAT- 1990/11/01 00:00
MHDA- 1990/11/01 00:01
CRDT- 1990/11/01 00:00
PHST- 1990/11/01 00:00 [pubmed]
PHST- 1990/11/01 00:01 [medline]
PHST- 1990/11/01 00:00 [entrez]
AID - 10.2527/1990.68113747x [doi]
PST - ppublish
SO  - J Anim Sci. 1990 Nov;68(11):3747-54. doi: 10.2527/1990.68113747x.