PMID- 21247708
OWN - NLM
STAT- MEDLINE
DCOM- 20110630
LR  - 20211203
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 207
IP  - 3
DP  - 2011 Mar 15
TI  - Serine threonine kinase 15 amplification in normal urothelium of cystectomy 
      specimens is no prognostic factor in urothelial carcinoma of the bladder.
PG  - 161-3
LID - 10.1016/j.prp.2010.12.006 [doi]
AB  - Predicting the prognosis of patients with bladder carcinoma is demanding, as 
      staging and grading alone do not suffice to make a precise assessment. Thus, the 
      prognostic relevance of various molecular factors was analyzed. It has recently 
      been postulated that gene amplification of serine threonine kinase 15 (STK15) in 
      normal urothelium is of prognostic value in muscle-invasive bladder cancer. The 
      aim of the present study was the validation of these findings. We retrospectively 
      analyzed amplification of STK15 gene in normal urothelium of 68 patients having 
      undergone radical cystectomy for urothelial bladder carcinoma of the bladder at 
      our department between 1998 and 2006 with available paraffin specimens. A tumor 
      micro array (TMA) was constructed, and fluorescence in situ hybridization (FISH) 
      was performed. Signals of chromosome 20 and STK15 gene were counted using 
      fluorescence microscopy. Specimens of 48 patients could be evaluated. The median 
      follow-up was 20 months [2-106 months]; disease-specific death occurred in 7 
      patients (15%). There was no amplification of STK15 gene in normal urothelium of 
      cystectomy specimens. In contrast to the preceding study, the present data show 
      no STK15 gene amplification in normal urothelium of cystectomy specimens. 
      Prediction of the oncological outcome warrants further research of different 
      markers.
CI  - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
FAU - Otto, Wolfgang
AU  - Otto W
AD  - Department of Urology, University of Regensburg, Caritas St. Josef Medical 
      Centre, Landshuterstrasse 65, 93053 Regensburg, Germany. 
      wolfgang.otto@klinik.uni-regensburg.de
FAU - Fritsche, Hans-Martin
AU  - Fritsche HM
FAU - Wild, Peter J
AU  - Wild PJ
FAU - Wieland, Wolf F
AU  - Wieland WF
FAU - Hartmann, Arndt
AU  - Hartmann A
FAU - Burger, Maximilian
AU  - Burger M
FAU - Hofstadter, Ferdinand
AU  - Hofstadter F
FAU - Denzinger, Stefan
AU  - Denzinger S
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20110117
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - EC 2.7.11.1 (AURKA protein, human)
RN  - EC 2.7.11.1 (Aurora Kinase A)
RN  - EC 2.7.11.1 (Aurora Kinases)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Aurora Kinase A
MH  - Aurora Kinases
MH  - Carcinoma/enzymology/*genetics/mortality/pathology/surgery
MH  - Chromosomes, Human, Pair 20
MH  - Cystectomy
MH  - Female
MH  - *Gene Amplification
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Neoplastic
MH  - Germany
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Microscopy, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Invasiveness
MH  - Protein Serine-Threonine Kinases/*genetics
MH  - Retrospective Studies
MH  - Survival Analysis
MH  - Time Factors
MH  - Tissue Array Analysis
MH  - Treatment Outcome
MH  - Urinary Bladder/*enzymology/pathology/surgery
MH  - Urinary Bladder Neoplasms/enzymology/*genetics/mortality/pathology/surgery
MH  - Urothelium/enzymology
EDAT- 2011/01/21 06:00
MHDA- 2011/07/01 06:00
CRDT- 2011/01/21 06:00
PHST- 2010/10/14 00:00 [received]
PHST- 2010/12/08 00:00 [accepted]
PHST- 2011/01/21 06:00 [entrez]
PHST- 2011/01/21 06:00 [pubmed]
PHST- 2011/07/01 06:00 [medline]
AID - S0344-0338(10)00291-8 [pii]
AID - 10.1016/j.prp.2010.12.006 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2011 Mar 15;207(3):161-3. doi: 10.1016/j.prp.2010.12.006. Epub 
      2011 Jan 17.