PMID- 21248284
OWN - NLM
STAT- MEDLINE
DCOM- 20110926
LR  - 20230119
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 84
IP  - 6
DP  - 2011 Jun
TI  - Peroxiredoxin 2 inhibits granulosa cell apoptosis during follicle atresia through 
      the NFKB pathway in mice.
PG  - 1182-9
LID - 10.1095/biolreprod.110.087569 [doi]
AB  - Peroxiredoxin 2 (PRDX2) has been known to act as an antioxidant enzyme whose main 
      function is H(2)O(2) reduction in cells. We aimed to study the expression 
      patterns of PRDX2 in mouse ovaries and explore the function of this protein in 
      apoptosis of granulosa cells (GCs). We found that the expression of the PRDX2 
      protein in atretic follicle GCs was markedly higher than in healthy follicle GCs. 
      In vitro, the transfection of siRNA targeting the Prdx2 gene inhibited the 
      proliferation and induced the apoptosis of primary cultured GCs. Furthermore, 
      suppression of PRDX2 resulted in the augmentation of endogenous H(2)O(2), and the 
      ability to eliminate the exogenous H(2)O(2) was attenuated. The expression of 
      PRDX2 and nuclear factor kappa-light-chain-enhancer of activated B cells (NFKB), 
      whose activity was inhibited by binding to IKB, increased in GCs treated with 
      various concentrations of H(2)O(2) for 30 min. However, no significant change in 
      cytoplasmic IKB expression was observed. At 2 h after treatment with H(2)O(2), 
      nuclear NFKB expression level was reduced, cytoplasmic IKB expression was 
      increased, and PRDX2 expression was unchanged. Silencing of the Prdx2 gene caused 
      early changes in NFKB and IKB expression in the primary cultured GCs compared to 
      that in control cells. Taken together, these data suggest that PRDX2 plays an 
      important role in inhibiting apoptosis in GCs and that PRDX2 actions may be 
      related to the expression of NFKB and IKB.
FAU - Yang, Shuhong
AU  - Yang S
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of 
      China.
FAU - Luo, Aiyue
AU  - Luo A
FAU - Hao, Xing
AU  - Hao X
FAU - Lai, Zhiwen
AU  - Lai Z
FAU - Ding, Ting
AU  - Ding T
FAU - Ma, Xiangyi
AU  - Ma X
FAU - Mayinuer, Maitituohe
AU  - Mayinuer M
FAU - Shen, Wei
AU  - Shen W
FAU - Wang, Xi
AU  - Wang X
FAU - Lu, Yunping
AU  - Lu Y
FAU - Ma, Ding
AU  - Ma D
FAU - Wang, Shixuan
AU  - Wang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110119
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Small Interfering)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.15 (Peroxiredoxins)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects/*physiology
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Gene Silencing
MH  - Granulosa Cells/cytology/*physiology
MH  - Hydrogen Peroxide/pharmacology
MH  - Mice
MH  - NF-kappa B/genetics/*metabolism
MH  - Ovarian Follicle/anatomy & histology/*physiology
MH  - Peroxiredoxins/genetics/*metabolism
MH  - RNA, Small Interfering
EDAT- 2011/01/21 06:00
MHDA- 2011/09/29 06:00
CRDT- 2011/01/21 06:00
PHST- 2011/01/21 06:00 [entrez]
PHST- 2011/01/21 06:00 [pubmed]
PHST- 2011/09/29 06:00 [medline]
AID - biolreprod.110.087569 [pii]
AID - 10.1095/biolreprod.110.087569 [doi]
PST - ppublish
SO  - Biol Reprod. 2011 Jun;84(6):1182-9. doi: 10.1095/biolreprod.110.087569. Epub 2011 
      Jan 19.