PMID- 21249212
OWN - NLM
STAT- MEDLINE
DCOM- 20110802
LR  - 20211020
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 6
IP  - 1
DP  - 2011 Jan 7
TI  - Dendritic cells transfected with scFv from Mab 7.B12 mimicking original antigen 
      gp43 induces protection against experimental Paracoccidioidomycosis.
PG  - e15935
LID - 10.1371/journal.pone.0015935 [doi]
LID - e15935
AB  - Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic 
      mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which 
      primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a 
      response in naive T cells, and they also participate in Th-cell education. 
      Furthermore, these cells have been used for therapy in several disease models. 
      Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain 
      variable fragment (scFv) of an anti-Id antibody that is capable of mimicking 
      gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were 
      immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein 
      was presented to the regional lymph nodes cells. Moreover, we showed that the DCs 
      transfected with scFv were capable of efficiently activating proliferation of 
      total lymph node cells and inducing a decrease in lung infection. Therefore, our 
      results suggested that the use of scFv-transfected DCs may be a promising therapy 
      in the paracoccidioidomycosis (PCM) model.
FAU - Ferreira, Karen S
AU  - Ferreira KS
AD  - Departamento de Ciencias Biologicas do Instituto de Ciencias Ambientais, Quimicas 
      e Farmaceuticas da Universidade Federal de Sao Paulo, Sao Paulo, Brazil. 
      karenspadari@gmail.com
FAU - Maranhao, Andrea Q
AU  - Maranhao AQ
FAU - Garcia, Maria C C
AU  - Garcia MC
FAU - Brigido, Marcelo M
AU  - Brigido MM
FAU - Santos, Suelen S
AU  - Santos SS
FAU - Lopes, Jose D
AU  - Lopes JD
FAU - Almeida, Sandro R
AU  - Almeida SR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110107
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (43 kDa protein, Paracoccidioides)
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Fungal)
RN  - 0 (Fungal Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (Single-Chain Antibodies)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/therapeutic use
MH  - Antibodies, Monoclonal/therapeutic use
MH  - Antigens, Fungal/*immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Fungal Proteins/*immunology
MH  - Glycoproteins/*immunology
MH  - Immunotherapy/*methods
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Mimicry
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*prevention & control/therapy
MH  - Single-Chain Antibodies/genetics/*therapeutic use
MH  - Transfection
PMC - PMC3017565
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2011/01/21 06:00
MHDA- 2011/08/04 06:00
PMCR- 2011/01/07
CRDT- 2011/01/21 06:00
PHST- 2010/09/22 00:00 [received]
PHST- 2010/12/02 00:00 [accepted]
PHST- 2011/01/21 06:00 [entrez]
PHST- 2011/01/21 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
PHST- 2011/01/07 00:00 [pmc-release]
AID - PONE-D-10-02722 [pii]
AID - 10.1371/journal.pone.0015935 [doi]
PST - epublish
SO  - PLoS One. 2011 Jan 7;6(1):e15935. doi: 10.1371/journal.pone.0015935.