PMID- 21256116
OWN - NLM
STAT- MEDLINE
DCOM- 20110406
LR  - 20211203
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 405
IP  - 3
DP  - 2011 Feb 18
TI  - Mild caloric restriction up-regulates the expression of prohibitin: a proteome 
      study.
PG  - 462-7
LID - 10.1016/j.bbrc.2011.01.054 [doi]
AB  - Caloric restriction (CR) is well known to expand lifespan in a variety of species 
      and to retard many age-related diseases. The effects of relatively mild CR on the 
      proteome profile in relation to lifespan have not yet been reported, despite the 
      more extensive studies of the stricter CR conditions. Thus, the present study was 
      conducted to elucidate the protein profiles in rat livers after mild CR for a 
      relatively short time. Young growing rats were fed CR diets (10% and 30% CR) for 
      1month. We performed the differential proteomic analysis of the rat livers using 
      two-dimensional electrophoresis combined with MALDI-TOF mass spectrometry. The 
      most remarkable protein among the differentially expressed proteins was found to 
      be prohibitin, the abundance of which was increased by 30% CR. Prohibitin is a 
      ubiquitously expressed protein shown to suppress cell proliferation and to be 
      related to longevity. The increase in prohibitin was observed both in 10% and 30% 
      CR by Western blot analysis. Furthermore, induction of AMP-activated kinase 
      (AMPK) protein, related to the actions of prohibitin in promoting longevity, was 
      observed. The increased prohibitin level in response to subtle CR suggests that 
      this increase may be one of the early events leading to the expansion of lifespan 
      in response to CR.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Takahashi, Shoko
AU  - Takahashi S
AD  - Department of Applied Biological Chemistry, The University of Tokyo, Tokyo, 
      Japan.
FAU - Masuda, Junko
AU  - Masuda J
FAU - Shimagami, Hiroshi
AU  - Shimagami H
FAU - Ohta, Yutaka
AU  - Ohta Y
FAU - Kanda, Tomomasa
AU  - Kanda T
FAU - Saito, Kenji
AU  - Saito K
FAU - Kato, Hisanori
AU  - Kato H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110120
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Prohibitins)
RN  - 0 (Proteome)
RN  - 0 (RNA, Messenger)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - Body Weight
MH  - *Caloric Restriction
MH  - Liver/anatomy & histology/metabolism
MH  - *Longevity
MH  - Male
MH  - Organ Size
MH  - Prohibitins
MH  - Proteome/*biosynthesis/genetics
MH  - Proteomics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Repressor Proteins/*biosynthesis/genetics
EDAT- 2011/01/25 06:00
MHDA- 2011/04/07 06:00
CRDT- 2011/01/25 06:00
PHST- 2011/01/12 00:00 [received]
PHST- 2011/01/15 00:00 [accepted]
PHST- 2011/01/25 06:00 [entrez]
PHST- 2011/01/25 06:00 [pubmed]
PHST- 2011/04/07 06:00 [medline]
AID - S0006-291X(11)00084-2 [pii]
AID - 10.1016/j.bbrc.2011.01.054 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Feb 18;405(3):462-7. doi: 
      10.1016/j.bbrc.2011.01.054. Epub 2011 Jan 20.