PMID- 21256457
OWN - NLM
STAT- MEDLINE
DCOM- 20110309
LR  - 20181201
IS  - 1878-1810 (Electronic)
IS  - 1878-1810 (Linking)
VI  - 157
IP  - 2
DP  - 2011 Feb
TI  - Bone marrow stromal cells as replacement cells for Parkinson's disease: 
      generation of an anatomical but not functional neuronal phenotype.
PG  - 56-63
LID - 10.1016/j.trsl.2010.11.001 [doi]
AB  - The focus of cell replacement therapies (CRTs) for Parkinson's disease has been 
      on delivering dopamine-producing cells to the striatum. Fetal grafts have proven 
      the feasibility of this approach, but an appropriate source of replacement cells 
      has restricted the clinical translation. Bone marrow stromal cells (BMSCs) have 
      been heralded as an ideal source of dopaminergic (DAergic) replacement cells, as 
      they are viewed as ethically acceptable, easily procured, and readily expanded. 
      It is known that they confer functional benefits, particularly in stroke models, 
      through the release of neurotrophic factors, but their transdifferentiation into 
      neurons is still under contention. We sought to evaluate the neuronal phenotype 
      and functional capacity of adult rat BMSCs after exposure to a novel multistep in 
      vitro differentiation protocol compared with cells exposed to other reported 
      neuronal differentiation conditions. We employed a systematic, comprehensive 
      method of assessment to determine the neuronal differentiation capacity of BMSCs. 
      Our fluorescence-activated cell sorting, immunofluorescent and semiquantitative 
      polymerase chain reaction results confirmed that undifferentiated BMSCs isolated 
      based on their adherence to plastic are of mesenchymal origin and express a range 
      of lineage markers. After exposure to preinduction and neuronal induction steps, 
      BMSCs down-regulate markers of other lineages but fail, as assessed by patch 
      clamp, to differentiate into functional neurons. Thus, for BMSCs to be considered 
      a source of DAergic neuronal replacement cells, their ability to 
      transdifferentiate terminally along a neuronal lineage first must be clarified 
      before attempting to direct more complex specification process required for them 
      to be used in Parkinson's-disease-focused CRTs.
CI  - Crown Copyright (c) 2011. Published by Mosby, Inc. All rights reserved.
FAU - Thomas, Meghan G
AU  - Thomas MG
AD  - Parkinson's Centre, Edith Cowan University, Perth, Western Australia; School of 
      Exercise Biomedical and Health Science, Edith Cowan University, Perth, Western 
      Australia, Australia. m.thomas@ecu.edu.au
FAU - Stone, Leah
AU  - Stone L
FAU - Evill, Lauren
AU  - Evill L
FAU - Ong, Syerna
AU  - Ong S
FAU - Ziman, Mel
AU  - Ziman M
FAU - Hool, Livia
AU  - Hool L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101205
PL  - United States
TA  - Transl Res
JT  - Translational research : the journal of laboratory and clinical medicine
JID - 101280339
SB  - IM
CIN - Transl Res. 2011 Feb;157(2):53-5. PMID: 21256456
MH  - Animals
MH  - *Bone Marrow Cells/cytology/metabolism
MH  - *Bone Marrow Transplantation
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Mesenchymal Stem Cells/cytology
MH  - Neurons/cytology/metabolism/*physiology
MH  - Parkinson Disease/*therapy
MH  - Phenotype
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/cytology/metabolism/*transplantation
EDAT- 2011/01/25 06:00
MHDA- 2011/03/10 06:00
CRDT- 2011/01/25 06:00
PHST- 2010/04/12 00:00 [received]
PHST- 2010/11/04 00:00 [revised]
PHST- 2010/11/04 00:00 [accepted]
PHST- 2011/01/25 06:00 [entrez]
PHST- 2011/01/25 06:00 [pubmed]
PHST- 2011/03/10 06:00 [medline]
AID - S1931-5244(10)00289-6 [pii]
AID - 10.1016/j.trsl.2010.11.001 [doi]
PST - ppublish
SO  - Transl Res. 2011 Feb;157(2):56-63. doi: 10.1016/j.trsl.2010.11.001. Epub 2010 Dec 
      5.