PMID- 21256693
OWN - NLM
STAT- MEDLINE
DCOM- 20110802
LR  - 20181201
IS  - 1879-0054 (Electronic)
IS  - 0739-7240 (Linking)
VI  - 40
IP  - 4
DP  - 2011 May
TI  - Tumor necrosis factor-alpha inhibits the stimulatory effect of luteinizing hormone 
      and prostaglandin E(2) on progesterone secretion by the bovine corpus luteum.
PG  - 183-91
LID - 10.1016/j.domaniend.2010.11.003 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) is involved in the tissue remodeling that occurs 
      in the corpus luteum (CL) during its development and regression. This cytokine is 
      also implicated in the regulation of reproduction by its actions on ovarian 
      steroidogenic cells. The aim of this study was to examine the influence of TNF-alpha 
      on (1) progesterone (P(4)) output by the bovine CL and on (2) the responsiveness 
      of the CL to LH or prostaglandin E(2) (PGE(2)) in vitro. In experiment 1, CL 
      (days 8 to 10 of the estrous cycle) were perfused by using an in vitro 
      microdialysis system with TNF-alpha (0.1, 0.5, or 1 mug/mL) alone or with TNF-alpha (1 
      mug/mL) followed by LH (1000 ng/mL) or PGE(2) (2 x 10(-5) M). Basal P(4) release 
      (P < 0.05) was increased by TNF-alpha (0.5 or 1 mug/mL). Moreover, TNF-alpha (1 mug/mL) 
      inhibited the stimulatory effect of LH or PGE(2) on P(4) output (P < 0.05). In 
      experiment 2, 4 h after intrauterine infusion of TNF-alpha (0.01 mug/mL or 1 mug/mL), 
      CL (days 8 to 10 of the estrous cycle) were collected by colpotomy, cultured, and 
      stimulated with LH (10 ng/mL) or PGE(2) (10(-6) M). Intrauterine infusion of 
      TNF-alpha at a concentration of 1 mug/mL increased basal P(4) output by CL (P < 0.05). 
      Moreover, the intrauterine infusion of TNF-alpha at a concentration of 0.01 mug/mL 
      inhibited the stimulatory effect of LH or PGE(2) on P(4) output (P < 0.05). These 
      results indicate that TNF-alpha (1) does not have an effect on the autonomous, 
      pulsatile release of P(4); (2) increases P(4) secretion by bovine CL with 
      increasing doses, and (3) reduces in a dose-dependent manner the responsiveness 
      of CL to luteotropic factors both directly (after infusion to CL) and indirectly 
      (after intrauterine infusion).
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Szostek, A Z
AU  - Szostek AZ
AD  - Department of Reproductive Immunology and Pathology, Institute of Animal 
      Reproduction and Food Research, Olsztyn, Poland.
FAU - Lukasik, K
AU  - Lukasik K
FAU - Majewska, M
AU  - Majewska M
FAU - Bah, M M
AU  - Bah MM
FAU - Znaniecki, R
AU  - Znaniecki R
FAU - Okuda, K
AU  - Okuda K
FAU - Skarzynski, D J
AU  - Skarzynski DJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101228
PL  - United States
TA  - Domest Anim Endocrinol
JT  - Domestic animal endocrinology
JID - 8505191
RN  - 0 (Estrogen Antagonists)
RN  - 0 (Hormone Antagonists)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Animals
MH  - Cattle/*physiology
MH  - Corpus Luteum/drug effects/*metabolism
MH  - Dinoprostone/pharmacology
MH  - Estrogen Antagonists/pharmacology
MH  - Estrous Cycle
MH  - Female
MH  - Hormone Antagonists/pharmacology
MH  - In Vitro Techniques
MH  - Luteinizing Hormone/*pharmacology
MH  - Progesterone/*metabolism
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 2011/01/25 06:00
MHDA- 2011/08/04 06:00
CRDT- 2011/01/25 06:00
PHST- 2010/07/25 00:00 [received]
PHST- 2010/09/21 00:00 [revised]
PHST- 2010/11/29 00:00 [accepted]
PHST- 2011/01/25 06:00 [entrez]
PHST- 2011/01/25 06:00 [pubmed]
PHST- 2011/08/04 06:00 [medline]
AID - S0739-7240(10)00142-6 [pii]
AID - 10.1016/j.domaniend.2010.11.003 [doi]
PST - ppublish
SO  - Domest Anim Endocrinol. 2011 May;40(4):183-91. doi: 
      10.1016/j.domaniend.2010.11.003. Epub 2010 Dec 28.