PMID- 21261017
OWN - NLM
STAT- MEDLINE
DCOM- 20111004
LR  - 20110124
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 24
IP  - 21
DP  - 2010 Nov
TI  - [Expression of CCR6 and CCR7 in laryngeal squamous cell carcinoma].
PG  - 975-9
AB  - OBJECTIVE: To evaluate the expressions of chemokine receptor 6 (CCR6), chemokine 
      receptor 7 (CCR7) and their ligands (CCL20, CCL19/CCL21) in laryngeal squamous 
      cell carcinoma (LSCC), and then explore their correlation with the 
      clinicopathological features of LSCC. METHOD: Blood samples, fresh specimens of 
      LSCC and paired adjacent tissues were collected. The expressions of CCR6, CCR7 
      and their ligands CCL20, CCL19/ CCL21 mRNA as well as the protein CCR6, CCR7 were 
      detected by real-time qRT-PCR and IHC respectively. Flow cytometry was also used 
      to investigate CCR6, CCR7 expressed on PBMC. RESULT: The relative expression 
      levels of CCR6, CCR7, CCL19 and CCL21 mRNA in tumor tissue was significantly 
      higher than that of adjacent tissues (P < 0.05), while the relative expression 
      level of CCL20 mRNA in tumor tissue were significantly lower than that of 
      adjacent tissues (P < 0.05). IHC confirmed the expression of protein CCR6 and 
      CCR7 in both tumor tissue and metastatic ILN and the expression levels of protein 
      CCR6, CCR7 were higher in the cases with lymphatic metastasis than that of those 
      without lymphatic metastasis (P < 0.05). FCM showed the percentage of CD4+ CCR6+ 
      T cells of LSCC was significantly higher than that of normal control (P < 0.05), 
      while that of CD4+ CCR7+ T cells was significantly lower (P < 0.05). CONCLUSION: 
      CCR6 and CCR7 are expressed in tumor situ, metastatic LN and PBMC,and might exert 
      a potential role in LSCC development.
FAU - Chen, Bin
AU  - Chen B
AD  - Department of Otolaryngology, Eye Ear Nose and Throat Hospital of Fudan 
      University, Shanghai 200031, China.
FAU - Lu, Liming
AU  - Lu L
FAU - Tao, Lei
AU  - Tao L
FAU - Zhou, Liang
AU  - Zhou L
FAU - Li, Shimin
AU  - Li S
FAU - Zhu, Li
AU  - Zhu L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (CCL19 protein, human)
RN  - 0 (CCL20 protein, human)
RN  - 0 (CCL21 protein, human)
RN  - 0 (CCR6 protein, human)
RN  - 0 (CCR7 protein, human)
RN  - 0 (Chemokine CCL19)
RN  - 0 (Chemokine CCL20)
RN  - 0 (Chemokine CCL21)
RN  - 0 (Receptors, CCR6)
RN  - 0 (Receptors, CCR7)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Chemokine CCL19/metabolism
MH  - Chemokine CCL20/metabolism
MH  - Chemokine CCL21/metabolism
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Middle Aged
MH  - Receptors, CCR6/*metabolism
MH  - Receptors, CCR7/*metabolism
EDAT- 2011/01/26 06:00
MHDA- 2011/10/05 06:00
CRDT- 2011/01/26 06:00
PHST- 2011/01/26 06:00 [entrez]
PHST- 2011/01/26 06:00 [pubmed]
PHST- 2011/10/05 06:00 [medline]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Nov;24(21):975-9.