PMID- 21261885
OWN - NLM
STAT- MEDLINE
DCOM- 20110518
LR  - 20211020
IS  - 1751-7915 (Electronic)
IS  - 1751-7915 (Print)
IS  - 1751-7915 (Linking)
VI  - 2
IP  - 1
DP  - 2009 Jan
TI  - Arabinoxylan-oligosaccharides (AXOS) affect the protein/carbohydrate fermentation 
      balance and microbial population dynamics of the Simulator of Human Intestinal 
      Microbial Ecosystem.
PG  - 101-13
LID - 10.1111/j.1751-7915.2008.00064.x [doi]
AB  - Arabinoxylan-oligosaccharides (AXOS) are a recently newly discovered class of 
      candidate prebiotics as - depending on their structure - they are fermented in 
      different regions of gastrointestinal tract. This can have an impact on the 
      protein/carbohydrate fermentation balance in the large intestine and, thus, 
      affect the generation of potentially toxic metabolites in the colon originating 
      from proteolytic activity. In this study, we screened different AXOS preparations 
      for their impact on the in vitro intestinal fermentation activity and microbial 
      community structure. Short-term fermentation experiments with AXOS with an 
      average degree of polymerization (avDP) of 29 allowed part of the 
      oligosaccharides to reach the distal colon, and decreased the concentration of 
      proteolytic markers, whereas AXOS with lower avDP were primarily fermented in the 
      proximal colon. Additionally, prolonged supplementation of AXOS with avDP 29 to 
      the Simulator of Human Intestinal Microbial Ecosystem (SHIME) reactor decreased 
      levels of the toxic proteolytic markers phenol and p-cresol in the two distal 
      colon compartments and increased concentrations of beneficial short-chain fatty 
      acids (SCFA) in all colon vessels (25-48%). Denaturant gradient gel 
      electrophoresis (DGGE) analysis indicated that AXOS supplementation only slightly 
      modified the total microbial community, implying that the observed effects on 
      fermentation markers are mainly caused by changes in fermentation activity. 
      Finally, specific quantitative PCR (qPCR) analysis showed that AXOS 
      supplementation significantly increased the amount of health-promoting 
      lactobacilli as well as of Bacteroides-Prevotella and Clostridium 
      coccoides-Eubacterium rectale groups. These data allow concluding that AXOS are 
      promising candidates to modulate the microbial metabolism in the distal colon.
CI  - Journal compilation (c) 2008 Society for Applied Microbiology and Blackwell 
      Publishing Ltd. No claim to original Belgium government works.
FAU - Sanchez, J I
AU  - Sanchez JI
AD  - Laboratory of Microbial Ecology and Technology (LabMET), Ghent University, Gent, 
      Belgium.
FAU - Marzorati, M
AU  - Marzorati M
FAU - Grootaert, C
AU  - Grootaert C
FAU - Baran, M
AU  - Baran M
FAU - Van Craeyveld, V
AU  - Van Craeyveld V
FAU - Courtin, C M
AU  - Courtin CM
FAU - Broekaert, W F
AU  - Broekaert WF
FAU - Delcour, J A
AU  - Delcour JA
FAU - Verstraete, W
AU  - Verstraete W
FAU - Van de Wiele, T
AU  - Van de Wiele T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20081013
PL  - United States
TA  - Microb Biotechnol
JT  - Microbial biotechnology
JID - 101316335
RN  - 0 (Oligosaccharides)
RN  - 0 (Prebiotics)
RN  - 0 (Proteins)
RN  - 0 (Xylans)
RN  - 9040-27-1 (arabinoxylan)
SB  - IM
MH  - Bacteria/genetics/isolation & purification/*metabolism
MH  - *Carbohydrate Metabolism
MH  - *Fermentation
MH  - Humans
MH  - Intestinal Mucosa/metabolism
MH  - Intestines/*microbiology
MH  - Models, Biological
MH  - Oligosaccharides/chemistry/*metabolism
MH  - Polymerization
MH  - Prebiotics/analysis
MH  - Proteins/*metabolism
MH  - Xylans/chemistry/*metabolism
PMC - PMC3815425
EDAT- 2009/01/01 00:00
MHDA- 2011/05/19 06:00
PMCR- 2009/01/01
CRDT- 2011/01/26 06:00
PHST- 2011/01/26 06:00 [entrez]
PHST- 2009/01/01 00:00 [pubmed]
PHST- 2011/05/19 06:00 [medline]
PHST- 2009/01/01 00:00 [pmc-release]
AID - 10.1111/j.1751-7915.2008.00064.x [doi]
PST - ppublish
SO  - Microb Biotechnol. 2009 Jan;2(1):101-13. doi: 10.1111/j.1751-7915.2008.00064.x. 
      Epub 2008 Oct 13.