PMID- 21262483
OWN - NLM
STAT- MEDLINE
DCOM- 20110504
LR  - 20110125
IS  - 1768-3238 (Electronic)
IS  - 1631-0691 (Linking)
VI  - 334
IP  - 1
DP  - 2011 Jan
TI  - Induced mutagenesis in Jatropha curcas L. using gamma rays and detection of DNA 
      polymorphism through RAPD marker.
PG  - 24-30
LID - 10.1016/j.crvi.2010.11.004 [doi]
AB  - The aim of this study is to examine the effect of different doses (control, 5, 
      10, 15, 20 and 25 Kr) of gamma irradiation on seed germination, flowering, fruit 
      and seed traits of Jatropha curcas and to identify DNA polymorphism among the 
      mutants through a Randomly Amplified Polymorphic DNA (RAPD) marker analysis. The 
      improved agronomic traits such as flowering, fruits and seeds were recorded in 5 
      Kr dose and seed germination percentage in 10 Kr dose treated plants, while 
      corresponding parameters were reduced significantly (P>0.05) in 25 Kr dose gamma 
      rays treated plants when compared to that of control. All the twenty-three random 
      primers used except six primers, namely OPAW16, OPAK07, OPAK15, OPS01, OPAK20 and 
      OPAL09 were showed polymorphic bands. The primers: OPAW16, OPAK07, OPAK15, OPS01, 
      OPAK20 and OPAL09 produced only one band each across the six mutants, while the 
      primers: OPU13, OPAB 15, OPF01 and OPAB11 were produced with maximum number of 
      bands (8). The number of amplicons varied from 1 to 8 with an average of 3.9 
      bands, of which 2.3 were polymorphic. The percentage of polymorphism per primer 
      ranged from 0 to 100 with an average of 55.16%. The Jaccard's coefficients of 
      dissimilarity varied from 0.324 to 0.397, indicative of the level of genetic 
      variation among the mutants studied. The maximum dissimilarity value (0.397) was 
      observed in 5 Kr mutant while the minimum value (0.250) was observed in 20 Kr 
      mutant when compared to that of control. In a dendrogram constructed based on 
      genetic similarity coefficients, the mutants were grouped into three main 
      clusters; (a) control, 10, 15 and 20 Kr dose mutants clustered together, (b) 25 
      Kr dose grouped alone, (c) 5 Kr dose also grouped alone. The mutants showing the 
      differences in morphological traits showed DNA polymorphism in PCR profile 
      amplified by RAPD marker. It is concluded that DNA polymorphism detected by RAPD 
      analysis offered a useful molecular marker for the identification of mutants in 
      gamma radiation treated plants.
CI  - Copyright (c) 2010 Academie des sciences. Published by Elsevier SAS. All rights 
      reserved.
FAU - Dhakshanamoorthy, Dharman
AU  - Dhakshanamoorthy D
AD  - Department of Botany, Annamalai University, Annamalainagar 608 002, Tamilnadu, 
      India.
FAU - Selvaraj, Radhakrishnan
AU  - Selvaraj R
FAU - Chidambaram, A L A
AU  - Chidambaram AL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101228
PL  - France
TA  - C R Biol
JT  - Comptes rendus biologies
JID - 101140040
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - DNA Primers
MH  - DNA, Plant/*genetics/isolation & purification/*radiation effects
MH  - Flowers/radiation effects
MH  - Fruit/genetics
MH  - *Gamma Rays
MH  - Genetic Markers
MH  - Germination/radiation effects
MH  - Jatropha/*genetics
MH  - Mutagenesis/*genetics/physiology
MH  - Polymorphism, Genetic/*genetics
MH  - Random Amplified Polymorphic DNA Technique
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seeds/genetics/radiation effects
EDAT- 2011/01/26 06:00
MHDA- 2011/05/05 06:00
CRDT- 2011/01/26 06:00
PHST- 2010/09/15 00:00 [received]
PHST- 2010/11/07 00:00 [revised]
PHST- 2010/11/10 00:00 [accepted]
PHST- 2011/01/26 06:00 [entrez]
PHST- 2011/01/26 06:00 [pubmed]
PHST- 2011/05/05 06:00 [medline]
AID - S1631-0691(10)00257-X [pii]
AID - 10.1016/j.crvi.2010.11.004 [doi]
PST - ppublish
SO  - C R Biol. 2011 Jan;334(1):24-30. doi: 10.1016/j.crvi.2010.11.004. Epub 2010 Dec 
      28.