PMID- 21263245
OWN - NLM
STAT- MEDLINE
DCOM- 20110301
LR  - 20110125
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 35
IP  - 2
DP  - 2011 Feb
TI  - Copy number variations and clinical outcome in atypical spitz tumors.
PG  - 243-52
LID - 10.1097/PAS.0b013e31820393ee [doi]
AB  - Atypical Spitz tumors (ASTs) are rare spitzoid neoplasms of uncertain biological 
      behavior. Our study was designed to characterize genetic abnormalities that may 
      help to differentiate ASTs from melanoma or Spitz nevi. We examined copy number 
      variation in formalin-fixed, paraffin-embedded samples using an Agilent 44k array 
      comparative genomic hybridization platform. Sixteen patients with AST (8 with 
      positive sentinel lymph node biopsy, 1 with distant metastasis), 8 patients with 
      Spitz nevi, and 3 patients with melanoma (2 spitzoid, 1 superficial spreading) 
      were evaluated. Chromosomal aberrations were found in 7 of 16 ASTs, 1 with fatal 
      outcome, 2 spitzoid melanomas, and 1 conventional melanoma. We found no 
      difference in chromosomal instability between AST patients with positive and 
      negative sentinel lymph node biopsies. Our patient with widely metastatic AST 
      lacked the most frequent aberrations in melanoma involving chromosomes 6 and 11q 
      that are loci targeted by fluorescence in situ hybridization (FISH) probes 
      developed to distinguish malignant melanoma from benign melanocytic lesions. The 
      vast majority of chromosomal abnormalities observed in ASTs are not commonly 
      found in melanomas, suggesting that AST may be a distinct clinical entity and 
      raising additional questions regarding their malignant potential, prognosis, and 
      clinical management. The current FISH probes failed to detect 1 spitzoid 
      melanoma, 1 fatal metastatic AST case, and the other chromosomally aberrant ASTs 
      in our series, but detected 1 spitzoid melanoma and 1 conventional melanoma. 
      Thus, a comprehensive, genome-wide approach to chromosomal abnormalities offered 
      greater sensitivity and specificity than current FISH probes in identifying 
      spitzoid lesions of uncertain malignant potential in this series.
FAU - Raskin, Leon
AU  - Raskin L
AD  - Department of Internal Medicine, University of Michigan, Ann Arbor, USA.
FAU - Ludgate, Mathew
AU  - Ludgate M
FAU - Iyer, Ramaswamy K
AU  - Iyer RK
FAU - Ackley, Todd E
AU  - Ackley TE
FAU - Bradford, Carol R
AU  - Bradford CR
FAU - Johnson, Timothy M
AU  - Johnson TM
FAU - Fullen, Douglas R
AU  - Fullen DR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization/methods
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - *Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Melanoma/genetics/secondary
MH  - Middle Aged
MH  - Nevus, Epithelioid and Spindle Cell/*genetics/pathology
MH  - Skin Neoplasms/*genetics/pathology
MH  - Young Adult
EDAT- 2011/01/26 06:00
MHDA- 2011/03/02 06:00
CRDT- 2011/01/26 06:00
PHST- 2011/01/26 06:00 [entrez]
PHST- 2011/01/26 06:00 [pubmed]
PHST- 2011/03/02 06:00 [medline]
AID - 00000478-201102000-00009 [pii]
AID - 10.1097/PAS.0b013e31820393ee [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2011 Feb;35(2):243-52. doi: 10.1097/PAS.0b013e31820393ee.