PMID- 21264061
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20231104
IS  - 1932-1058 (Electronic)
IS  - 1932-1058 (Print)
IS  - 1932-1058 (Linking)
VI  - 4
IP  - 4
DP  - 2010 Dec 6
TI  - Simple surface modification of poly(dimethylsiloxane) for DNA hybridization.
PG  - 46504
LID - 10.1063/1.3523055 [doi]
LID - 046504
AB  - Here, we present a simple chemical modification of poly(dimethylsiloxane) (PDMS) 
      by curing a mixture of 2 wt% undecylenic acid (UDA) in PDMS prepolymer on a 
      gold-coated glass slide. This gold slide had been previously pretreated with a 
      self-assembled hydrophilic monolayer of 3-mercaptopropionic acid (MPA). During 
      curing of the UDA∕PDMS prepolymer, the hydrophilic UDA carboxyl moieties diffuses 
      toward the hydrophilic MPA carboxyl moieties on the gold surface. This diffusion 
      of the UDA within the PDMS prepolymer to the surface is a direct result of 
      surface energy minimization. Once completely cured, the PDMS is peeled off the 
      gold substrate, thereby exposing the interfacial carboxyl groups. These groups 
      are then available for subsequent attachment of 5(')-amino terminated DNA 
      oligonucleotides via amide linkages. Our results show that the covalently 
      tethered oligonucleotides can successfully capture fluorescein-labeled 
      complementary oligonucleotides via hybridization, which are visualized using 
      fluorescence microscopy.
FAU - Zhou, Jinwen
AU  - Zhou J
AD  - Centre for NanoScale Science and Nanotechnology, School of Chemical and Physical 
      Sciences, Flinders University, Adelaide SA 5001, Australia.
FAU - Voelcker, Nicolas H
AU  - Voelcker NH
FAU - Ellis, Amanda V
AU  - Ellis AV
LA  - eng
PT  - Journal Article
DEP - 20101206
PL  - United States
TA  - Biomicrofluidics
JT  - Biomicrofluidics
JID - 101293825
PMC - PMC3025499
EDAT- 2011/01/26 06:00
MHDA- 2011/01/26 06:01
PMCR- 2010/12/06
CRDT- 2011/01/26 06:00
PHST- 2010/09/05 00:00 [received]
PHST- 2010/11/03 00:00 [accepted]
PHST- 2011/01/26 06:00 [entrez]
PHST- 2011/01/26 06:00 [pubmed]
PHST- 2011/01/26 06:01 [medline]
PHST- 2010/12/06 00:00 [pmc-release]
AID - 014004BMF [pii]
AID - 10.1063/1.3523055 [doi]
PST - epublish
SO  - Biomicrofluidics. 2010 Dec 6;4(4):46504. doi: 10.1063/1.3523055.