PMID- 21268094
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20211203
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 112
IP  - 2
DP  - 2011 Feb
TI  - Positive regulation of inositol 1,4,5-trisphosphate-induced Ca2+ release by 
      mammalian target of rapamycin (mTOR) in RINm5F cells.
PG  - 723-33
LID - 10.1002/jcb.23006 [doi]
AB  - The inositol 1,4,5-trisphosphate receptor (IP(3)R), a ligand-gated Ca(2+) 
      channel, is the main regulator of intracellular Ca(2+) mobilization in 
      non-excitable cells. An emerging body of evidence suggests that specific 
      regulatory control of the Ca(2+) signaling pathway is modulated by the activation 
      of additional signaling pathways. In the present study, we investigated the 
      influence of the PI3-kinase/mammalian target of rapamycin (mTOR) pathway on the 
      activity of the IP(3)R/Ca(2+) signaling pathway in RINm5F cells. We used a 
      co-immunoprecipitation approach to show that mTOR physically interacts with 
      IP(3)R-3 in an mTOR activity-dependent manner. We also showed that IP(3)R is 
      phosphorylated by mTOR in cellulo. All the conditions known to modulate mTOR 
      activity (IGF-1, wortmannin, rapamycin, PP242, and nutrient starvation) were 
      shown to modify carbachol-induced Ca(2+) signaling in RINm5F cells. Lastly, we 
      used an assay that directly measures the activity of IP(3)R, to show that mTOR 
      increases the apparent affinity of IP(3)R. Given that mTOR controls cell 
      proliferation and cell homeostasis, and that Ca(2+) plays a key role in these two 
      phenomena, it follows that mTOR facilitates IP(3)R-mediated Ca(2+) release when 
      the nutritional status of cells requires it.
CI  - Copyright (c) 2011 Wiley-Liss, Inc.
FAU - Fregeau, Marc-Olivier
AU  - Fregeau MO
AD  - Faculty of Medicine and Health Sciences, Department of Pharmacology, Universite 
      de Sherbrooke, Sherbrooke, Quebec, Canada.
FAU - Regimbald-Dumas, Yannik
AU  - Regimbald-Dumas Y
FAU - Guillemette, Gaetan
AU  - Guillemette G
LA  - eng
GR  - Canadian Institutes of Health Research/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Inositol 1,4,5-Trisphosphate Receptors)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Calcium/*metabolism
MH  - Cell Line, Tumor
MH  - Immunoprecipitation
MH  - Inositol 1,4,5-Trisphosphate/*pharmacology
MH  - Inositol 1,4,5-Trisphosphate Receptors/metabolism
MH  - Phosphorylation
MH  - Protein Binding
MH  - Rats
MH  - TOR Serine-Threonine Kinases/*metabolism
EDAT- 2011/01/27 06:00
MHDA- 2011/05/04 06:00
CRDT- 2011/01/27 06:00
PHST- 2011/01/27 06:00 [entrez]
PHST- 2011/01/27 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
AID - 10.1002/jcb.23006 [doi]
PST - ppublish
SO  - J Cell Biochem. 2011 Feb;112(2):723-33. doi: 10.1002/jcb.23006.