PMID- 21268245
OWN - NLM
STAT- MEDLINE
DCOM- 20110420
LR  - 20191210
IS  - 1615-9314 (Electronic)
IS  - 1615-9306 (Linking)
VI  - 34
IP  - 3
DP  - 2011 Feb
TI  - High-performance liquid chromatographic determination of memantine hydrochloride 
      in rat plasma using sensitive fluorometric derivatization.
PG  - 241-6
LID - 10.1002/jssc.201000579 [doi]
AB  - In this study, we investigated a simple, sensitive and reliable liquid 
      chromatography-fluorescence detection method for the determination of memantine 
      hydrochloride in rat plasma which was based on derivatization with 
      9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was 
      introduced into derivatization of memantine hydrochloride in rat plasma. The 
      amino groups of memantine hydrochloride and amantadine hydrochloride (internal 
      standard) were trapped with FMOC-Cl to form memantine hydrochloride-FMOC-Cl and 
      amantadine hydrochloride-FMOC-Cl compositions, which can be very compatible for 
      LC-FLD. Precipitation of plasma proteins by acetonitrile was followed by vortex 
      mixing and centrifugation. Chromatographic separation was performed on a C(18) 
      column (DIAMONSIL 150 x 4.6 mm, id 5 mum) with a mobile phase consisting of 
      acetonitrile and water at a flow rate of 1.0 mL/min. The retention times of 
      memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions 
      were 23.69 and 40.27 min, respectively. Optimal conditions for the derivatization 
      of memantine hydrochloride were also described. The limit of quantification (LOQ) 
      was 25 ng/mL for memantine hydrochloride in plasma, the linear range was 
      0.025-5.0 mug/mL in plasma with a correlation coefficient (r) of 0.9999. The 
      relative standard deviations (RSDs) of intra-day and inter-day assays were 
      4.46-12.19 and 5.23-11.50%, respectively. The validated method was successfully 
      applied to the determination of memantine hydrochloride in rat plasma samples.
CI  - Copyright (c) 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Xie, Mei-Fen
AU  - Xie MF
AD  - Department of Pharmaceutical Analysis, School of Pharmacy, Fudan University, 
      Shanghai, PR China.
FAU - Zhou, Wei
AU  - Zhou W
FAU - Tong, Xin-Yi
AU  - Tong XY
FAU - Chen, Yi-Le
AU  - Chen YL
FAU - Cai, Yi
AU  - Cai Y
FAU - Li, Yan
AU  - Li Y
FAU - Duan, Geng-Li
AU  - Duan GL
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110107
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Dopamine Agents)
RN  - 0 (Fluorenes)
RN  - 9PLB0BTT90 (1-(9-fluorenyl)methyl chloroformate)
RN  - W8O17SJF3T (Memantine)
SB  - IM
MH  - Alzheimer Disease/blood
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dopamine Agents/*blood/chemistry
MH  - Fluorenes/chemistry
MH  - Humans
MH  - Memantine/*blood/chemistry
MH  - Rats
MH  - Rats, Wistar
EDAT- 2011/01/27 06:00
MHDA- 2011/04/22 06:00
CRDT- 2011/01/27 06:00
PHST- 2010/08/02 00:00 [received]
PHST- 2010/11/10 00:00 [revised]
PHST- 2010/11/10 00:00 [accepted]
PHST- 2011/01/27 06:00 [entrez]
PHST- 2011/01/27 06:00 [pubmed]
PHST- 2011/04/22 06:00 [medline]
AID - 10.1002/jssc.201000579 [doi]
PST - ppublish
SO  - J Sep Sci. 2011 Feb;34(3):241-6. doi: 10.1002/jssc.201000579. Epub 2011 Jan 7.