PMID- 21269456
OWN - NLM
STAT- MEDLINE
DCOM- 20110503
LR  - 20211020
IS  - 1743-422X (Electronic)
IS  - 1743-422X (Linking)
VI  - 8
DP  - 2011 Jan 26
TI  - Japanese encephalitis Virus wild strain infection suppresses dendritic cells 
      maturation and function, and causes the expansion of regulatory T cells.
PG  - 39
LID - 10.1186/1743-422X-8-39 [doi]
AB  - BACKGROUND: Japanese encephalitis (JE) caused by Japanese encephalitis virus 
      (JEV) accounts for acute illness and death. However, few studies have been 
      conducted to unveil the potential pathogenesis mechanism of JEV. Dendritic cells 
      (DCs) are the most prominent antigen-presenting cells (APCs) which induce dual 
      humoral and cellular responses. Thus, the investigation of the interaction 
      between JEV and DCs may be helpful for resolving the mechanism of viral escape 
      from immune surveillance and JE pathogenesis. RESULTS: We examined the 
      alterations of phenotype and function of DCs including bone marrow-derived DCs 
      (bmDCs) in vitro and spleen-derived DCs (spDCs) in vivo due to JEV P3 wild strain 
      infection. Our results showed that JEV P3 infected DCs in vitro and in vivo. The 
      viral infection inhibited the expression of cell maturation surface markers 
      (CD40, CD80 and CD83) and MHCⅠ, and impaired the ability of P3-infected DCs for 
      activating allogeneic naive T cells. In addition, P3 infection suppressed the 
      expression of interferon (IFN)-alpha and tumor necrosis factor (TNF)-alpha but enhanced 
      the production of chemokine (C-C motif) ligand 2 (CCL2) and interleukin (IL)-10 
      of DCs. The infected DCs expanded the population of CD4+ Foxp3+ regulatory T cell 
      (Treg). CONCLUSION: JEV P3 infection of DCs impaired cell maturation and T cell 
      activation, modulated cytokine productions and expanded regulatory T cells, 
      suggesting a possible mechanism of JE development.
FAU - Cao, Shengbo
AU  - Cao S
AD  - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural 
      University, Wuhan, Hubei 430070, PR China.
FAU - Li, Yaoming
AU  - Li Y
FAU - Ye, Jing
AU  - Ye J
FAU - Yang, Xiaohong
AU  - Yang X
FAU - Chen, Long
AU  - Chen L
FAU - Liu, Xueqin
AU  - Liu X
FAU - Chen, Huanchun
AU  - Chen H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110126
PL  - England
TA  - Virol J
JT  - Virology journal
JID - 101231645
RN  - 0 (Antigens, CD)
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/*virology
MH  - Encephalitis Virus, Japanese/*immunology/*pathogenicity
MH  - Histocompatibility Antigens Class I/analysis
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes, Regulatory/*immunology/*virology
PMC - PMC3038949
EDAT- 2011/01/29 06:00
MHDA- 2011/05/04 06:00
PMCR- 2011/01/26
CRDT- 2011/01/29 06:00
PHST- 2010/10/26 00:00 [received]
PHST- 2011/01/26 00:00 [accepted]
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/05/04 06:00 [medline]
PHST- 2011/01/26 00:00 [pmc-release]
AID - 1743-422X-8-39 [pii]
AID - 10.1186/1743-422X-8-39 [doi]
PST - epublish
SO  - Virol J. 2011 Jan 26;8:39. doi: 10.1186/1743-422X-8-39.