PMID- 21270231
OWN - NLM
STAT- MEDLINE
DCOM- 20110718
LR  - 20211020
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 49
IP  - 4
DP  - 2011 Apr
TI  - Utility of a panviral microarray for detection of swine respiratory viruses in 
      clinical samples.
PG  - 1542-8
LID - 10.1128/JCM.01876-10 [doi]
AB  - Several factors have recently converged, elevating the need for highly parallel 
      diagnostic platforms that have the ability to detect many known, novel, and 
      emerging pathogenic agents simultaneously. Panviral DNA microarrays represent the 
      most robust approach for massively parallel viral surveillance and detection. The 
      Virochip is a panviral DNA microarray that is capable of detecting all known 
      viruses, as well as novel viruses related to known viral families, in a single 
      assay and has been used to successfully identify known and novel viral agents in 
      clinical human specimens. However, the usefulness and the sensitivity of the 
      Virochip platform have not been tested on a set of clinical veterinary specimens 
      with the high degree of genetic variance that is frequently observed with swine 
      virus field isolates. In this report, we investigate the utility and sensitivity 
      of the Virochip to positively detect swine viruses in both cell culture-derived 
      samples and clinical swine samples. The Virochip successfully detected porcine 
      reproductive and respiratory syndrome virus (PRRSV) in serum containing 6.10 x 
      10(2) viral copies per microliter and influenza A virus in lung lavage fluid 
      containing 2.08 x 10(6) viral copies per microliter. The Virochip also 
      successfully detected porcine circovirus type 2 (PCV2) in serum containing 2.50 x 
      10(8) viral copies per microliter and porcine respiratory coronavirus (PRCV) in 
      turbinate tissue homogenate. Collectively, the data in this report demonstrate 
      that the Virochip can successfully detect pathogenic viruses frequently found in 
      swine in a variety of solid and liquid specimens, such as turbinate tissue 
      homogenate and lung lavage fluid, as well as antemortem samples, such as serum.
FAU - Nicholson, Tracy L
AU  - Nicholson TL
AD  - Virus and Prion Research Unit, National Animal Disease Center, Agricultural 
      Research Service, USDA, Ames, IA 50010, USA. tracy.nicholson@ars.usda.gov
FAU - Kukielka, Deborah
AU  - Kukielka D
FAU - Vincent, Amy L
AU  - Vincent AL
FAU - Brockmeier, Susan L
AU  - Brockmeier SL
FAU - Miller, Laura C
AU  - Miller LC
FAU - Faaberg, Kay S
AU  - Faaberg KS
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20110126
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Animals
MH  - Circovirus/isolation & purification
MH  - Clinical Laboratory Techniques/*methods
MH  - Influenza A virus/isolation & purification
MH  - Microarray Analysis/*methods
MH  - Porcine Respiratory Coronavirus/isolation & purification
MH  - Porcine respiratory and reproductive syndrome virus/isolation & purification
MH  - Respiratory Tract Infections/diagnosis/*veterinary/virology
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
MH  - Virology/*methods
MH  - Virus Diseases/diagnosis/*veterinary/virology
PMC - PMC3122799
EDAT- 2011/01/29 06:00
MHDA- 2011/07/19 06:00
PMCR- 2011/10/01
CRDT- 2011/01/29 06:00
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/07/19 06:00 [medline]
PHST- 2011/10/01 00:00 [pmc-release]
AID - JCM.01876-10 [pii]
AID - 1876-10 [pii]
AID - 10.1128/JCM.01876-10 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2011 Apr;49(4):1542-8. doi: 10.1128/JCM.01876-10. Epub 2011 Jan 
      26.