PMID- 21270950
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20110714
LR  - 20220330
IS  - 1874-3250 (Electronic)
IS  - 1874-3250 (Linking)
VI  - 5
DP  - 2011 Jan 7
TI  - Osteogenic predifferentiation of human bone marrow-derived stem cells by 
      short-term mechanical stimulation.
PG  - 1-6
LID - 10.2174/1874325001105010001 [doi]
AB  - It is commonly accepted that bone marrow-derived stem cells (BMSCs) have to be 
      expanded in vitro, but a prolonged time in culture decreases their multilineage 
      potential. Mechanical and biological stimuli have been used to improve their 
      osteogenic potential. While long-term stimulation has been shown to improve 
      osteogenic differentiation, it remains to be seen whether short-term stimulation 
      is also sufficient.We investigated the influence of 24 hours' cyclic loading 
      (0.05Hz, 4kPa) on gene expression of human BMSCs in three-dimensional fibrin-DMEM 
      constructs (n=7) in a compression bioreactor using DNA-array technology. 
      Expression of the following genes showed a significant increase after mechanical 
      stimulation: 2.6-fold osteopontin (OPN) and integrin-beta1 (ITGB1), 2.2-fold 
      transforming growth factor-beta-receptor 1 (TGF-beta-R1) and 2.4-fold SMAD5 expression, 
      compared to controls without mechanical stimulation (p<0.05 each). 
      Platelet-derived growth factor-alpha (PDGF-alpha ) and annexin-V were also significantly 
      overexpressed, the mechanical stimulation resulting in a 1.8-fold and 1.6-fold 
      expression (p<0.05).Cells were identified as osteoblast precursors with a high 
      proliferative capacity. Given the identical in-vitro environment for both groups, 
      the increase in gene expression has been interpreted as a direct influence of 
      cyclic mechanical stimulation on osteogenic differentiation. It may be postulated 
      that short-term mechanical stimulation results in an improved osseous integration 
      of tissue engineered grafts in bone defect healing.
FAU - Matziolis, Doerte
AU  - Matziolis D
AD  - Orthopaedic Department, Center for Musculoskeletal Surgery.
FAU - Tuischer, Jens
AU  - Tuischer J
FAU - Matziolis, Georg
AU  - Matziolis G
FAU - Kasper, Grit
AU  - Kasper G
FAU - Duda, Georg
AU  - Duda G
FAU - Perka, Carsten
AU  - Perka C
LA  - eng
PT  - Journal Article
DEP - 20110107
PL  - United Arab Emirates
TA  - Open Orthop J
JT  - The open orthopaedics journal
JID - 101480491
PMC - PMC3027083
OTO - NOTNLM
OT  - Osteogenic predifferentiation
OT  - bioreactor.
OT  - human bone marrow-derived stem cells
OT  - mechanical stimulation
EDAT- 2011/01/29 06:00
MHDA- 2011/01/29 06:01
PMCR- 2011/01/01
CRDT- 2011/01/29 06:00
PHST- 2010/04/21 00:00 [received]
PHST- 2010/07/08 00:00 [revised]
PHST- 2010/07/19 00:00 [accepted]
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/01/29 06:01 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - TOORTHJ-5-1 [pii]
AID - 10.2174/1874325001105010001 [doi]
PST - epublish
SO  - Open Orthop J. 2011 Jan 7;5:1-6. doi: 10.2174/1874325001105010001.