PMID- 21272268
OWN - NLM
STAT- MEDLINE
DCOM- 20110729
LR  - 20110415
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 33
IP  - 3
DP  - 2011 Jun
TI  - Various patterns of IgH deletion identified by FISH using combined IgH and 
      IgH/CCND1 probes in multiple myeloma and chronic lymphocytic leukemia.
PG  - 299-304
LID - 10.1111/j.1751-553X.2010.01290.x [doi]
AB  - INTRODUCTION: Interphase fluorescence in situ hybridization (FISH) can identify 
      submicroscopic deletions adjacent to the breakpoints of rearrangements undetected 
      by conventional cytogenetics. In this study, the characteristics and frequency of 
      the IgH deletion identified by interphase FISH were investigated in patients with 
      multiple myeloma (MM) and chronic lymphocytic leukemia (CLL). METHODS: The study 
      group included 29 patients with MM and eight patients with CLL. Interphase FISH 
      was performed with the IgH dual color, break-apart rearrangement probe and the 
      IgH/CCND1 dual color, dual fusion translocation probe. RESULTS: The IgH deletion 
      was found in 14% (4/29) of patients with MM and 13% (1/8) of the patients with 
      CLL. Four patients had deletions of the whole or variable region of IgH on the 
      native chromosome 14, whereas one patient had a deletion of the IgH variable 
      region on a der(11)t(11;14). In two patients, the IgH break-apart FISH showed 
      both patterns with and without IgH deletions. In cases showing the same pattern 
      by IgH break-apart FISH, the IgH/CCND1 FISH showed different patterns, and vice 
      versa. CONCLUSION: A variety of patterns of the IgH deletion were identified by 
      interphase FISH using IgH break-apart and IgH/CCND1 probes in patients with MM 
      and CLL. The results of this study suggest that the integrated information 
      obtained with IgH break-apart and IgH/CCND1 FISH was needed to interpret FISH 
      results unambiguously.
CI  - (c) 2011 Blackwell Publishing Ltd.
FAU - Hwang, Y
AU  - Hwang Y
AD  - Department of Laboratory Medicine, Ewha Womans University School of Medicine, 
      Seoul, Korea.
FAU - Lee, J Y
AU  - Lee JY
FAU - Mun, Y C
AU  - Mun YC
FAU - Seong, C M
AU  - Seong CM
FAU - Chung, W S
AU  - Chung WS
FAU - Huh, J
AU  - Huh J
LA  - eng
PT  - Journal Article
DEP - 20110128
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Aged
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Cyclin D1/genetics
MH  - Female
MH  - *Gene Deletion
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - *In Situ Hybridization, Fluorescence
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/*genetics
MH  - Translocation, Genetic
EDAT- 2011/01/29 06:00
MHDA- 2011/07/30 06:00
CRDT- 2011/01/29 06:00
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/07/30 06:00 [medline]
AID - 10.1111/j.1751-553X.2010.01290.x [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2011 Jun;33(3):299-304. doi: 10.1111/j.1751-553X.2010.01290.x. 
      Epub 2011 Jan 28.