PMID- 21272311
OWN - NLM
STAT- MEDLINE
DCOM- 20110509
LR  - 20211020
IS  - 1471-2156 (Electronic)
IS  - 1471-2156 (Linking)
VI  - 12
DP  - 2011 Jan 27
TI  - An integrated molecular cytogenetic map of Cucumis sativus L. chromosome 2.
PG  - 18
LID - 10.1186/1471-2156-12-18 [doi]
AB  - BACKGROUND: Integration of molecular, genetic and cytological maps is still a 
      challenge for most plant species. Recent progress in molecular and cytogenetic 
      studies created a basis for developing integrated maps in cucumber (Cucumis 
      sativus L.). RESULTS: In this study, eleven fosmid clones and three plasmids 
      containing 45S rDNA, the centromeric satellite repeat Type III and the 
      pericentriomeric repeat CsRP1 sequences respectively were hybridized to cucumber 
      metaphase chromosomes to assign their cytological location on chromosome 2. 
      Moreover, an integrated molecular cytogenetic map of cucumber chromosomes 2 was 
      constructed by fluorescence in situ hybridization (FISH) mapping of 11 fosmid 
      clones together with the cucumber centromere-specific Type III sequence on 
      meiotic pachytene chromosomes. The cytogenetic map was fully integrated with 
      genetic linkage map since each fosmid clone was anchored by a genetically mapped 
      simple sequence repeat marker (SSR). The relationship between the genetic and 
      physical distances along chromosome was analyzed. CONCLUSIONS: Recombination was 
      not evenly distributed along the physical length of chromosome 2. Suppression of 
      recombination was found in centromeric and pericentromeric regions. Our results 
      also indicated that the molecular markers composing the linkage map for 
      chromosome 2 provided excellent coverage of the chromosome.
FAU - Han, Yonghua
AU  - Han Y
AD  - National Maize Improvement Center of China, Key Laboratory of Crop Genetic 
      Improvement and Genome of Ministry of Agriculture, Beijing Key Laboratory of Crop 
      Genetic Improvement, China Agricultural University, Beijing 100094, PR China. 
      yhhan@genetics.ac.cn
FAU - Zhang, Zhonghua
AU  - Zhang Z
FAU - Huang, Sanwen
AU  - Huang S
FAU - Jin, Weiwei
AU  - Jin W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110127
PL  - England
TA  - BMC Genet
JT  - BMC genetics
JID - 100966978
SB  - IM
MH  - Centromere
MH  - *Chromosome Mapping
MH  - Chromosomes, Plant
MH  - Cucumis sativus/*genetics
MH  - In Situ Hybridization, Fluorescence
PMC - PMC3039625
EDAT- 2011/01/29 06:00
MHDA- 2011/05/10 06:00
PMCR- 2011/01/27
CRDT- 2011/01/29 06:00
PHST- 2010/11/27 00:00 [received]
PHST- 2011/01/27 00:00 [accepted]
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/05/10 06:00 [medline]
PHST- 2011/01/27 00:00 [pmc-release]
AID - 1471-2156-12-18 [pii]
AID - 10.1186/1471-2156-12-18 [doi]
PST - epublish
SO  - BMC Genet. 2011 Jan 27;12:18. doi: 10.1186/1471-2156-12-18.