PMID- 21274603
OWN - NLM
STAT- MEDLINE
DCOM- 20111229
LR  - 20220801
IS  - 1573-4994 (Electronic)
IS  - 1053-0509 (Linking)
VI  - 21
IP  - 4
DP  - 2011 Jul
TI  - Detection of Staphylococcus aureus carrying the gene for toxic shock syndrome 
      toxin 1 by quantum-dot-probe complexes.
PG  - 1525-30
LID - 10.1007/s10895-011-0840-4 [doi]
AB  - In this study, a high-sensitive and high-specific method to detect the toxic 
      shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus was developed 
      based on quantum dot (QD) and oligonucleotide probe complexes. S. aureus carrying 
      tst gene which is responsible for the production of TSST-1 were detected based on 
      fluorescence resonance energy transfer (FRET) occurring between CdSe/ZnS QD 
      donors and black hole quencher (BHQ) acceptors. QD-DNA probe was prepared by 
      conjugating the carboxyl-modified QD and the amino-modified DNA with the EDC. 
      Photoluminescence (PL) quenching was achieved through FRET after the addition of 
      BHQ-DNA which was attached to tst gene probe by match sequence hybridization. The 
      PL recovery was detected in the presence of target DNA by BHQ-DNA detached from 
      QD-DNA probe because of the different affinities. In contrast, mismatch 
      oligonucleotides and DNAs of other bacteria did not contribute to fluorescence 
      intensity recovery, which exhibits the higher selectivity of the biosensor. The 
      experimental results showed clearly that the intensity of recovered QD PL is 
      linear to the concentration of target DNA within the range of 0.2-1.2 muM and the 
      detection limit was 0.2 muM.
CI  - (c) Springer Science+Business Media, LLC 2011
FAU - Wang, Dan
AU  - Wang D
AD  - The Co-construction Laboratory of Microbial Molecular Biology of Province 
      Department and Ministry of Science and Technology, College of Life Science, Hunan 
      Normal University, Changsha, Hunan 410081, People's Republic of China.
FAU - Chen, Hui
AU  - Chen H
FAU - Li, Hua
AU  - Li H
FAU - He, QiZhi
AU  - He Q
FAU - Ding, XingHua
AU  - Ding X
FAU - Deng, Le
AU  - Deng L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110128
PL  - Netherlands
TA  - J Fluoresc
JT  - Journal of fluorescence
JID - 9201341
RN  - 0 (Bacterial Toxins)
RN  - 0 (Cadmium Compounds)
RN  - 0 (Enterotoxins)
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (Selenium Compounds)
RN  - 0 (Sulfides)
RN  - 0 (Superantigens)
RN  - 0 (Zinc Compounds)
RN  - 0 (enterotoxin F, Staphylococcal)
RN  - A7F646JC5C (cadmium selenide)
RN  - KPS085631O (zinc sulfide)
SB  - IM
MH  - Bacterial Toxins/*analysis
MH  - Cadmium Compounds/*chemistry
MH  - Enterotoxins/*analysis
MH  - Fluorescence Resonance Energy Transfer
MH  - Luminescence
MH  - Oligonucleotide Probes/*chemistry
MH  - *Quantum Dots
MH  - Selenium Compounds/*chemistry
MH  - Spectrometry, Fluorescence
MH  - Staphylococcus aureus/*genetics
MH  - Sulfides/*chemistry
MH  - Superantigens/*analysis
MH  - Zinc Compounds/*chemistry
EDAT- 2011/01/29 06:00
MHDA- 2011/12/30 06:00
CRDT- 2011/01/29 06:00
PHST- 2010/10/28 00:00 [received]
PHST- 2011/01/10 00:00 [accepted]
PHST- 2011/01/29 06:00 [entrez]
PHST- 2011/01/29 06:00 [pubmed]
PHST- 2011/12/30 06:00 [medline]
AID - 10.1007/s10895-011-0840-4 [doi]
PST - ppublish
SO  - J Fluoresc. 2011 Jul;21(4):1525-30. doi: 10.1007/s10895-011-0840-4. Epub 2011 Jan 
      28.