PMID- 21277027
OWN - NLM
STAT- MEDLINE
DCOM- 20110708
LR  - 20231213
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 140
IP  - 3-4
DP  - 2011 Apr 15
TI  - Evaluation of the immunogenicity of a transgenic tobacco plant expressing the 
      recombinant fusion protein of GP5 of porcine reproductive and respiratory 
      syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pigs.
PG  - 215-25
LID - 10.1016/j.vetimm.2011.01.002 [doi]
AB  - Escherichia coli heat-labile enterotoxin B subunit (LTB) can be used as an 
      adjuvant for co-administered antigens. Our previous study showed that the 
      expression of neutralizing epitope GP5 of porcine reproductive and respiratory 
      syndrome virus (PRRSV) in transgenic tobacco plant (GP5-T) could induce 
      PRRSV-specific immune responses in pigs. A transgenic tobacco plant co-expressing 
      LTB and PRRSV GP5 as a fusion protein (LTB-GP5-T) was further constructed and its 
      immunogenicity was evaluated. Pigs were given orally three consecutive doses of 
      equal concentration of recombinant GP5 protein expressed in leaves of LTB-GP5-T 
      or GP5-T at a 2-week interval and challenged with PRRSV at 7 weeks post-initial 
      immunization. Pigs receiving LTB-GP5-T or GP5-T developed PRRSV-specific 
      antibody- and cell-mediated immunity and showed significantly lower viremia and 
      tissue viral load and milder lung lesions than wild type tobacco plant (W-T). The 
      LTB-GP5-T-treated group had relatively higher immune responses than the 
      GP5-T-treated group, although the differences were not statistically significant.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Chia, Min-Yuan
AU  - Chia MY
AD  - Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, Taipei 
      106, Taiwan, ROC.
FAU - Hsiao, Shih-Hsuan
AU  - Hsiao SH
FAU - Chan, Hui-Ting
AU  - Chan HT
FAU - Do, Yi-Yin
AU  - Do YY
FAU - Huang, Pung-Ling
AU  - Huang PL
FAU - Chang, Hui-Wen
AU  - Chang HW
FAU - Tsai, Yi-Chieh
AU  - Tsai YC
FAU - Lin, Chun-Ming
AU  - Lin CM
FAU - Pang, Victor Fei
AU  - Pang VF
FAU - Jeng, Chian-Ren
AU  - Jeng CR
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110111
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA Primers)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Vaccines, Edible)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Adjuvants, Immunologic/administration & dosage
MH  - Administration, Oral
MH  - Animals
MH  - Antibodies, Viral/blood/metabolism
MH  - Bacterial Toxins/administration & dosage/*genetics/*immunology
MH  - Base Sequence
MH  - DNA Primers/genetics
MH  - Enterotoxins/administration & dosage/*genetics/*immunology
MH  - Escherichia coli/genetics/immunology
MH  - Escherichia coli Proteins/administration & dosage/*genetics/*immunology
MH  - Lymphocyte Activation
MH  - Male
MH  - Plant Leaves/immunology
MH  - Plants, Genetically Modified
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/prevention & 
      control/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/*immunology/pathogenicity
MH  - Recombinant Fusion Proteins/genetics/immunology
MH  - Saliva/immunology
MH  - Sus scrofa
MH  - Swine
MH  - Nicotiana/*genetics/*immunology
MH  - Vaccines, Edible/administration & dosage/genetics
MH  - Vaccines, Synthetic/administration & dosage/genetics
MH  - Viral Envelope Proteins/administration & dosage/*genetics/*immunology
MH  - Viral Load
MH  - Viral Vaccines/administration & dosage/genetics
EDAT- 2011/02/01 06:00
MHDA- 2011/07/09 06:00
CRDT- 2011/02/01 06:00
PHST- 2010/08/04 00:00 [received]
PHST- 2010/11/12 00:00 [revised]
PHST- 2011/01/01 00:00 [accepted]
PHST- 2011/02/01 06:00 [entrez]
PHST- 2011/02/01 06:00 [pubmed]
PHST- 2011/07/09 06:00 [medline]
AID - S0165-2427(11)00004-3 [pii]
AID - 10.1016/j.vetimm.2011.01.002 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2011 Apr 15;140(3-4):215-25. doi: 
      10.1016/j.vetimm.2011.01.002. Epub 2011 Jan 11.