PMID- 21277991
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20211203
IS  - 1878-5875 (Electronic)
IS  - 1357-2725 (Linking)
VI  - 43
IP  - 5
DP  - 2011 May
TI  - Mammalian Ste20-like protein kinase 3 plays a role in hypoxia-induced apoptosis 
      of trophoblast cell line 3A-sub-E.
PG  - 742-50
LID - 10.1016/j.biocel.2011.01.015 [doi]
AB  - Mammalian Ste20-like protein kinase 3 (Mst3) is a key player in inducing 
      apoptosis in a variety of cell types and has recently been shown to participate 
      in the signaling pathway of hypoxia-induced apoptosis of human trophoblast cell 
      line 3A-sub-E (3A). It is believed that oxidative stress may occur during hypoxia 
      and induce the expression of Mst3 in 3A cells via the activation of c-Jun 
      N-terminal protein kinase 1 (JNK1). This hypothesis was demonstrated by the 
      suppressive effect of dl-alpha-lipoic acid, a reactive oxygen species scavenger, in 
      hypoxia-induced responses of 3A cells such as Mst3 expression, nitrotyrosine 
      formation, JNK1 activation and apoptosis. Similar results were also observed in 
      trophoblasts of human placental explants in both immunohistochemical studies and 
      immunoblot analyses. These suggested that the activation of Mst3 might trigger 
      the apoptotic process in trophoblasts by activating caspase 3 and possibly other 
      apoptotic pathways. The role of nitric oxide synthase (NOS) and NADPH oxidase 
      (NOX) in hypoxia-induced Mst3 up-regulation was also demonstrated by the 
      inhibitory effect of N(G)-nitro-l-arginine and apocynin, which inhibits NOS and 
      NOX, respectively. Oxidative stress was postulated to be induced by NOS and NOX 
      in 3A cells during hypoxia. In conclusion, hypoxia induces oxidative stress in 
      human trophoblasts by activating NOS and NOX. Subsequently, Mst3 is up-regulated 
      and plays an important role in hypoxia-induced apoptosis of human trophoblasts.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Wu, Hung-Yi
AU  - Wu HY
AD  - Department of Biological Science and Technology, National Chiao Tung University, 
      75 Po Ai Street, Hsinchu 300, Taiwan, ROC.
FAU - Lin, Chia-Ying
AU  - Lin CY
FAU - Chen, Tai-Chang
AU  - Chen TC
FAU - Pan, Shien-Tung
AU  - Pan ST
FAU - Yuan, Chiun-Jye
AU  - Yuan CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110126
PL  - Netherlands
TA  - Int J Biochem Cell Biol
JT  - The international journal of biochemistry & cell biology
JID - 9508482
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 2.7.1.- (STK24 protein, human)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 8)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - *Apoptosis
MH  - Caspase 3/metabolism
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Female
MH  - Humans
MH  - Mitogen-Activated Protein Kinase 8/metabolism
MH  - NADPH Oxidases/metabolism
MH  - Nitric Oxide Synthase/metabolism
MH  - Oxidative Stress
MH  - Placenta/cytology
MH  - Pregnancy
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Signal Transduction
MH  - Trophoblasts/*cytology/*enzymology/metabolism
MH  - Up-Regulation
EDAT- 2011/02/01 06:00
MHDA- 2011/08/02 06:00
CRDT- 2011/02/01 06:00
PHST- 2010/11/19 00:00 [received]
PHST- 2011/01/18 00:00 [revised]
PHST- 2011/01/19 00:00 [accepted]
PHST- 2011/02/01 06:00 [entrez]
PHST- 2011/02/01 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
AID - S1357-2725(11)00032-X [pii]
AID - 10.1016/j.biocel.2011.01.015 [doi]
PST - ppublish
SO  - Int J Biochem Cell Biol. 2011 May;43(5):742-50. doi: 
      10.1016/j.biocel.2011.01.015. Epub 2011 Jan 26.