PMID- 21278380
OWN - NLM
STAT- MEDLINE
DCOM- 20110609
LR  - 20211020
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 286
IP  - 12
DP  - 2011 Mar 25
TI  - ATF2 interacts with beta-cell-enriched transcription factors, MafA, Pdx1, and 
      beta2, and activates insulin gene transcription.
PG  - 10449-56
LID - 10.1074/jbc.M110.209510 [doi]
AB  - Pancreatic beta-cell-restricted expression of insulin is established through several 
      critical cis-regulatory elements located in the insulin gene promoter region. The 
      principal cis elements are A-boxes, E1, and C1/RIPE3b. The beta-cell-enriched 
      transcription factors Pdx1 and Beta2 bind to the A-boxes and E1 element, 
      respectively. A beta-cell-specific trans-acting factor binding to C1/RIPE3b (termed 
      RIPE3b1 activator) was detected by electrophoretic mobility shift assay and has 
      been identified as MafA, a member of the Maf family of basic leucine zipper 
      (bZip) proteins. Here, ATF2, a member of the ATF/CREB family of basic leucine 
      zipper proteins, was identified as a component of the RIPE3b1 activator. ATF2 
      alone was unable to bind to the C1/RIPE3b element but acquired binding capacity 
      upon complex formation with MafA. ATF2 also interacted with Pdx1 and Beta2, and 
      co-expression of ATF2, MafA, Pdx1, and Beta2 resulted in a synergistic activation 
      of the insulin promoter. Immunohistochemical analysis of mouse pancreas tissue 
      sections showed that ATF2 is enriched in islet endocrine cells, including 
      beta-cells. RNAi-mediated knockdown of MafA or ATF2 in the MIN6 beta-cell line resulted 
      in a significant decrease in endogenous levels of insulin mRNA. These data 
      indicate that ATF2 is an essential component of the positive regulators of the 
      insulin gene expression.
FAU - Han, Song-iee
AU  - Han SI
AD  - Laboratory of Molecular and Developmental Biology, Graduate School of Biological 
      Science, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
FAU - Yasuda, Kunio
AU  - Yasuda K
FAU - Kataoka, Kohsuke
AU  - Kataoka K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110128
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Atf2 protein, mouse)
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Insulin)
RN  - 0 (MAFA protein, human)
RN  - 0 (Maf Transcription Factors, Large)
RN  - 0 (Mafa protein, mouse)
RN  - 0 (NEUROD1 protein, human)
RN  - 0 (Neurod1 protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Trans-Activators)
RN  - 0 (pancreatic and duodenal homeobox 1 protein)
SB  - IM
MH  - Activating Transcription Factor 2/genetics/*metabolism
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/genetics/*metabolism
MH  - Gene Expression Regulation/physiology
MH  - Gene Knockdown Techniques
MH  - HeLa Cells
MH  - Homeodomain Proteins/genetics/*metabolism
MH  - Humans
MH  - Insulin/*biosynthesis/genetics
MH  - Insulin-Secreting Cells/cytology/*metabolism
MH  - Maf Transcription Factors, Large/genetics/*metabolism
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Protein Binding
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Response Elements/physiology
MH  - Trans-Activators/genetics/*metabolism
MH  - Transcription, Genetic/*physiology
PMC - PMC3060498
EDAT- 2011/02/01 06:00
MHDA- 2011/06/10 06:00
PMCR- 2012/03/25
CRDT- 2011/02/01 06:00
PHST- 2011/02/01 06:00 [entrez]
PHST- 2011/02/01 06:00 [pubmed]
PHST- 2011/06/10 06:00 [medline]
PHST- 2012/03/25 00:00 [pmc-release]
AID - S0021-9258(20)53870-2 [pii]
AID - M110.209510 [pii]
AID - 10.1074/jbc.M110.209510 [doi]
PST - ppublish
SO  - J Biol Chem. 2011 Mar 25;286(12):10449-56. doi: 10.1074/jbc.M110.209510. Epub 
      2011 Jan 28.