PMID- 2127906
OWN - NLM
STAT- MEDLINE
DCOM- 19910507
LR  - 20190720
IS  - 0008-4166 (Print)
IS  - 0008-4166 (Linking)
VI  - 36
IP  - 12
DP  - 1990 Dec
TI  - Degradation of dimethyl nitrosamine by Methylosinus trichosporium OB3b.
PG  - 834-8
AB  - The degradation of dimethyl nitrosoamine (DMNA) by a methanotroph, Methylosinus 
      trichosporium OB3b, was studied using 14C-labelled DMNA. The organism was capable 
      of assimilating DMNA-carbon and converting it to CO2. The rates of CO2 production 
      (VCO2) from DMNA and its cellular uptake (VP) were linearly correlated with DMNA 
      concentrations of 0.03-10 mM, which corresponded to approximately 3% of added 
      DMNA metabolized in 24 h. These rates were two to three orders of magnitude less 
      than the rate of uptake of methane (VCH4. VCH4 was suppressed when the 
      concentrations of DMNA exceeded 0.3 mM. In the presence of 0.1 mM DMNA, VP and 
      VCO2 were essentially the same in the presence or absence of methane in the first 
      8 h of incubation, but declined sharply thereafter only when methane was absent. 
      These observations suggest that the metabolism of DMNA was carried out by methane 
      monooxygenase (MMO), and that NADH, a cofactor for MMO, may be provided by the 
      oxidation of the stored compounds in the cells when methane is not available.
FAU - Yoshinari, T
AU  - Yoshinari T
AD  - Wadsworth Center for Laboratories and Research, New York State Department of 
      Health, Albany 12201-0509.
FAU - Shafer, D
AU  - Shafer D
LA  - eng
GR  - R23 ES 02060/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - Can J Microbiol
JT  - Canadian journal of microbiology
JID - 0372707
RN  - 142M471B3J (Carbon Dioxide)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.14.13.25 (methane monooxygenase)
RN  - M43H21IO8R (Dimethylnitrosamine)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Carbon Dioxide/metabolism
MH  - Dimethylnitrosamine/*metabolism
MH  - Methane/metabolism
MH  - Methylococcaceae/enzymology/*metabolism
MH  - Oxidation-Reduction
MH  - Oxygenases/metabolism
EDAT- 1990/12/01 00:00
MHDA- 1990/12/01 00:01
CRDT- 1990/12/01 00:00
PHST- 1990/12/01 00:00 [pubmed]
PHST- 1990/12/01 00:01 [medline]
PHST- 1990/12/01 00:00 [entrez]
AID - 10.1139/m90-144 [doi]
PST - ppublish
SO  - Can J Microbiol. 1990 Dec;36(12):834-8. doi: 10.1139/m90-144.