PMID- 21281194
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20220409
IS  - 0002-9645 (Print)
IS  - 1943-5681 (Electronic)
IS  - 0002-9645 (Linking)
VI  - 72
IP  - 2
DP  - 2011 Feb
TI  - Comparison of five blood-typing methods for the feline AB blood group system.
PG  - 203-9
LID - 10.2460/ajvr.72.2.203 [doi]
AB  - Objective-To compare the ease of use and accuracy of 5 feline AB blood-typing 
      methods: card agglutination (CARD), immunochromatographic cartridge (CHROM), 
      gel-based (GEL), and conventional slide (SLIDE) and tube (TUBE) agglutination 
      assays. Sample Population-490 anticoagulated blood samples from sick and healthy 
      cats submitted to the Transfusion or Clinical Laboratory at the Veterinary 
      Hospital of the University of Pennsylvania. Procedures-Sample selection was 
      purposely biased toward those from anemic, type B, or type AB cats or those with 
      autoagglutination. All blood samples were tested by use of GEL, SLIDE, and TUBE 
      methods. Fifty-eight samples were also tested by use of CARD and CHROM methods. 
      The presence of alloantibodies in all cats expressing the B antigen as detected 
      by use of any method was also assessed. Results-Compared with the historical 
      gold-standard TUBE method, good to excellent agreement was achieved with the 
      other typing tests: CARD, 53 of 58 (91% agreement); CHROM, 55 of 58 (95%); GEL, 
      487 of 490 (99%); and SLIDE, 482 of 487 (99%; 3 samples were excluded because of 
      autoagglutination). Four of the samples with discordant test results originated 
      from cats with FeLV-related anemia. Conclusions and Clinical Relevance-Current 
      laboratory and in-clinic methods provide simple and accurate typing for the 
      feline AB blood group system with few discrepancies. Retyping after in-clinic 
      typing with the GEL or TUBE laboratory methods is recommended to confirm any type 
      B or AB cats.
FAU - Seth, Mayank
AU  - Seth M
AD  - Section of Medical Genetics, School of Veterinary Medicine, University of 
      Pennsylvania, Philadelphia, PA 19104, USA.
FAU - Jackson, Karen V
AU  - Jackson KV
FAU - Giger, Urs
AU  - Giger U
LA  - eng
GR  - P40 RR002512/RR/NCRR NIH HHS/United States
GR  - P40 RR002512-27/RR/NCRR NIH HHS/United States
GR  - RR02512/RR/NCRR NIH HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Blood Group Antigens)
SB  - IM
MH  - Animals
MH  - Blood Group Antigens
MH  - Blood Grouping and Crossmatching/methods/*veterinary
MH  - Cats/*blood
PMC - PMC3115202
MID - NIHMS269999
EDAT- 2011/02/02 06:00
MHDA- 2011/03/30 06:00
PMCR- 2011/06/15
CRDT- 2011/02/02 06:00
PHST- 2011/02/02 06:00 [entrez]
PHST- 2011/02/02 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
PHST- 2011/06/15 00:00 [pmc-release]
AID - 10.2460/ajvr.72.2.203 [doi]
PST - ppublish
SO  - Am J Vet Res. 2011 Feb;72(2):203-9. doi: 10.2460/ajvr.72.2.203.